PAI-1 antibody
Rabbit anti Human PAI-1 antibody recognises human PAI-1, also known as plasminogen activator inhibitor-1 or SERPINE1. PAI-1 is a ~45 kDa serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis.
Mutations of the SERPINE1 gene are associated with Plasminogen activator inhibitor-1 deficiency (PAI-1D), a condition characterized by episodes of severe hemorrhage (Saes et al. 2019). Heterozygous carriers of the mutation do not display the same phenotype, instead demonstrate longer teleomere length, lower incidence of diabetes and increased longevity (Khan et al. 2017).
Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and very-low-density lipoprotein (Altalhi et al. 2021).
Mutations of the SERPINE1 gene are associated with Plasminogen activator inhibitor-1 deficiency (PAI-1D), a condition characterized by episodes of severe hemorrhage (Saes et al. 2019). Heterozygous carriers of the mutation do not display the same phenotype, instead demonstrate longer teleomere length, lower incidence of diabetes and increased longevity (Khan et al. 2017).
Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and very-low-density lipoprotein (Altalhi et al. 2021).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antiserum to human PAI-1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human PAI-1
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05121
- Entrez Gene
- SERPINE1
- GO Terms
- GO:0005886 plasma membrane
- GO:0001300 chronological cell aging
- GO:0002020 protease binding
- GO:0002576 platelet degranulation
- GO:0004252 serine-type endopeptidase activity
- GO:0004867 serine-type endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0050829 defense response to Gram-negative bacterium
- GO:0010469 regulation of receptor activity
- GO:0010757 negative regulation of plasminogen activation
- GO:0014912 negative regulation of smooth muscle cell migration
- GO:0030168 platelet activation
- GO:0031012 extracellular matrix
- GO:0031093 platelet alpha granule lumen
- GO:0032757 positive regulation of interleukin-8 production
- GO:0033629 negative regulation of cell adhesion mediated by integrin
- GO:0035491 positive regulation of leukotriene production involved in inflammatory response
- GO:0042730 fibrinolysis
- GO:0043066 negative regulation of apoptosis
- GO:0045766 positive regulation of angiogenesis
- GO:0048260 positive regulation of receptor-mediated endocytosis
- GO:0051918 negative regulation of fibrinolysis
- GO:0061044 negative regulation of vascular wound healing
- GO:0070887 cellular response to chemical stimulus
- GO:0071222 cellular response to lipopolysaccharide
- GO:0090026 positive regulation of monocyte chemotaxis
- GO:2000098 negative regulation of smooth muscle cell-matrix adhesion
- Regulatory
- For research purposes only
Applications of PAI-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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