PAI-1 antibody

Rabbit anti Human PAI-1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
PAI-1

Product Code Applications Pack Size List Price Your Price Qty
AHP1617
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg loader
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loader
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Rabbit anti Human PAI-1 antibody recognises human PAI-1, also known as plasminogen activator inhibitor-1 or SERPINE1. PAI-1 is a ~45 kDa serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis.

Mutations of the SERPINE1 gene are associated with Plasminogen activator inhibitor-1 deficiency (PAI-1D), a condition characterized by episodes of severe hemorrhage (Saes et al. 2019). Heterozygous carriers of the mutation do not display the same phenotype, instead demonstrate longer teleomere length, lower incidence of diabetes and increased longevity (Khan et al. 2017).

Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and very-low-density lipoprotein (Altalhi et al. 2021).

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to human PAI-1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human PAI-1
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Synonyms
SERPINE1
RRID
AB_2187035
UniProt
P05121
Entrez Gene
SERPINE1
GO Terms
GO:0005886 plasma membrane
GO:0001300 chronological cell aging
GO:0002020 protease binding
GO:0002576 platelet degranulation
GO:0004252 serine-type endopeptidase activity
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0050829 defense response to Gram-negative bacterium
GO:0010469 regulation of receptor activity
GO:0010757 negative regulation of plasminogen activation
GO:0014912 negative regulation of smooth muscle cell migration
GO:0030168 platelet activation
GO:0031012 extracellular matrix
GO:0031093 platelet alpha granule lumen
GO:0032757 positive regulation of interleukin-8 production
GO:0033629 negative regulation of cell adhesion mediated by integrin
GO:0035491 positive regulation of leukotriene production involved in inflammatory response
GO:0042730 fibrinolysis
GO:0043066 negative regulation of apoptosis
GO:0045766 positive regulation of angiogenesis
GO:0048260 positive regulation of receptor-mediated endocytosis
GO:0051918 negative regulation of fibrinolysis
GO:0061044 negative regulation of vascular wound healing
GO:0070887 cellular response to chemical stimulus
GO:0071222 cellular response to lipopolysaccharide
GO:0090026 positive regulation of monocyte chemotaxis
GO:2000098 negative regulation of smooth muscle cell-matrix adhesion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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