Rabbit anti p27/Kip1 (pThr187) antibody
recognizes p27/Kip1, also known as cyclin-dependent kinase inhibitor p27 or cyclin-dependent kinase inhibitor 1B, when phosphorylated at threonine 187. p27/Kip1 is a cyclin-dependent kinase inhibitor (CKI) that belongs to the Cip/Kip protein family (Cerqueira et al. 2014
p27/Kip1 inhibits cyclin-dependent kinases (CDKs) and cyclin-CDK complexes; playing a role in the regulation of cell cycle progression at G1 (Cerqueira et al.
2014). Phosphorylation of p27/Kip1 at threonine 187 by CDK2 leads to the ubiquitination and subsequent degradation of p27/Kip by the 26S proteosome (Das et al. 2016
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to p27/Kip1 (pThr187) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 187 of human p27/Kip1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
regulation of cyclin-dependent protein kinase activity
G1 phase of mitotic cell cycle
G1/S transition of mitotic cell cycle
S phase of mitotic cell cycle
cyclin-dependent protein kinase inhibitor activity
transforming growth factor beta receptor, cytoplasmic mediator activity
induction of apoptosis
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
cell cycle arrest
autophagic cell death
negative regulation of cell proliferation
protein phosphatase binding
negative regulation of cell growth
negative regulation of gene-specific transcription
nerve growth factor receptor signaling pathway
cellular response to lithium ion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of p27/Kip1 (pThr187) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Cerqueira, A. et al. (2014) Genetic characterization of the role of the Cip/Kip family of proteins as cyclin-dependent kinase inhibitors and assembly factors.
Mol Cell Biol. 34 (8): 1452-9.
Das, R.K. et al. (2016) Cryptic sequence features within the disordered protein p27Kip1 regulate cell cycle signaling.
Proc Natl Acad Sci U S A. 113 (20): 5616-21.
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