p27/Kip1 (pThr187) antibody

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Rabbit anti p27/Kip1 (pThr187)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2662 P WB 50 µl
Rabbit anti p27/Kip1 (pThr187) antibody recognizes p27/Kip1, also known as cyclin-dependent kinase inhibitor p27 or cyclin-dependent kinase inhibitor 1B, when phosphorylated at threonine 187. p27/Kip1 is a cyclin-dependent kinase inhibitor (CKI) that belongs to the Cip/Kip protein family (Cerqueira et al. 2014).

p27/Kip1 inhibits cyclin-dependent kinases (CDKs) and cyclin-CDK complexes; playing a role in the regulation of cell cycle progression at G1 (Cerqueira et al. 2014). Phosphorylation of p27/Kip1 at threonine 187 by CDK2 leads to the ubiquitination and subsequent degradation of p27/Kip by the 26S proteosome (Das et al. 2016).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to p27/Kip1 (pThr187) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 187 of human p27/Kip1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P46527 Related reagents
Entrez Gene
CDKN1B Related reagents
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005829 cytosol
GO:0004861 cyclin-dependent protein kinase inhibitor activity
GO:0005072 transforming growth factor beta receptor, cytoplasmic mediator activity
GO:0005654 nucleoplasm
GO:0006917 induction of apoptosis
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0007050 cell cycle arrest
GO:0048102 autophagic cell death
GO:0008285 negative regulation of cell proliferation
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030308 negative regulation of cell growth
GO:0032582 negative regulation of gene-specific transcription
GO:0048011 nerve growth factor receptor signaling pathway
GO:0071285 cellular response to lithium ion
Regulatory
For research purposes only

Applications of p27/Kip1 (pThr187) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Cerqueira, A. et al. (2014) Genetic characterization of the role of the Cip/Kip family of proteins as cyclin-dependent kinase inhibitors and assembly factors.
    Mol Cell Biol. 34 (8): 1452-9.
  2. Das, R.K. et al. (2016) Cryptic sequence features within the disordered protein p27Kip1 regulate cell cycle signaling.
    Proc Natl Acad Sci U S A. 113 (20): 5616-21.
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