p27/Kip1 (pThr187) antibody
Rabbit anti p27/Kip1 (pThr187) antibody recognizes p27/Kip1, also known as cyclin-dependent kinase inhibitor p27 or cyclin-dependent kinase inhibitor 1B, when phosphorylated at threonine 187. p27/Kip1 is a cyclin-dependent kinase inhibitor (CKI) that belongs to the Cip/Kip protein family (Cerqueira et al. 2014).
p27/Kip1 inhibits cyclin-dependent kinases (CDKs) and cyclin-CDK complexes; playing a role in the regulation of cell cycle progression at G1 (Cerqueira et al. 2014). Phosphorylation of p27/Kip1 at threonine 187 by CDK2 leads to the ubiquitination and subsequent degradation of p27/Kip by the 26S proteosome (Das et al. 2016).
p27/Kip1 inhibits cyclin-dependent kinases (CDKs) and cyclin-CDK complexes; playing a role in the regulation of cell cycle progression at G1 (Cerqueira et al. 2014). Phosphorylation of p27/Kip1 at threonine 187 by CDK2 leads to the ubiquitination and subsequent degradation of p27/Kip by the 26S proteosome (Das et al. 2016).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to p27/Kip1 (pThr187) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding threonine 187 of human p27/Kip1
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P46527
- Entrez Gene
- CDKN1B
- GO Terms
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0005829 cytosol
- GO:0004861 cyclin-dependent protein kinase inhibitor activity
- GO:0005072 transforming growth factor beta receptor, cytoplasmic mediator activity
- GO:0005654 nucleoplasm
- GO:0006917 induction of apoptosis
- GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
- GO:0007050 cell cycle arrest
- GO:0048102 autophagic cell death
- GO:0008285 negative regulation of cell proliferation
- GO:0019903 protein phosphatase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030308 negative regulation of cell growth
- GO:0032582 negative regulation of gene-specific transcription
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0071285 cellular response to lithium ion
- Regulatory
- For research purposes only
Applications of p27/Kip1 (pThr187) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/50 | 1/100 | |
| Western Blotting | 1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Cerqueira, A. et al. (2014) Genetic characterization of the role of the Cip/Kip family of proteins as cyclin-dependent kinase inhibitors and assembly factors.
Mol Cell Biol. 34 (8): 1452-9. -
Das, R.K. et al. (2016) Cryptic sequence features within the disordered protein p27Kip1 regulate cell cycle signaling.
Proc Natl Acad Sci U S A. 113 (20): 5616-21.
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