Non-Neuronal Enolase antibody

Rabbit anti Human Non-Neuronal Enolase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Format
Purified
Specificity
Non-Neuronal Enolase
Region
(ALPHA-ALPHA)

Product Code Applications Pack Size List Price Your Price Qty
6880-0410
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E P WB 1 ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Human non-neuronal enolase polyclonal antibody recognises human non-neuronal enolase, also known as alpha enolase or enolase 1. Rabbit anti non-neuronal enolase demostrates less than 0.1% cross-reactivity with neuron-specific enolase.

Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. The gene coding for the 48 kDa form of non-neuronal enolase also codes for a shorter 37 kDa isoform, myc-binding protein 1 (MBP-1) lacking the N-terminal 93 amino acids of non-neuronal enolase.
PrecisionAb Antibodies

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Native human NNE
Approx. Protein Concentrations
IgG concentration 5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 1/25 1/100
Immunohistology - Paraffin 1/25 1/100
Western Blotting

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:FITC
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

References for Non-Neuronal Enolase antibody

  1. Wang, W. et al. (2005) Identification of alpha-enolase as a nuclear DNA-binding protein in the zona fasciculata but not the zona reticularis of the human adrenal cortex.
    J Endocrinol. 184: 85-94.
  2. Prunotto, M. et al. (2010) Endocellular polyamine availability modulates epithelial-to-mesenchymal transition and unfolded protein response in MDCK cells.
    Lab Invest. 90: 929-39.
  3. Kimura Y et al. (2016) Circulating antibodies to α-enolase and phospholipase A2 receptor and composition of glomerular deposits in Japanese patients with primary or secondary membranous nephropathy.
    Clin Exp Nephrol. Feb 1. [Epub ahead of print]
  4. Garcia, A. et al. (2014) Immunohistochemical evaluation of the goat forestomach during prenatal development.
    J Vet Sci. 15 (1): 35-43.

Immunohistology - Paraffin

Synonyms
NNE
RRID
AB_620318
UniProt
P06733
Entrez Gene
ENO1
Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products