Non-Neuronal Enolase antibody
siRNA Validated
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IC WB ResetRabbit anti Human Non-Neuronal Enolase
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- Non-Neuronal Enolase
- Region
- (ALPHA-ALPHA)
Quick Links:
Antibody Quality and 
Unrivaled 
| Rabbit anti Human non-neuronal enolase polyclonal antibody recognises human non-neuronal enolase, also known as alpha enolase or enolase 1. Rabbit anti non-neuronal enolase demostrates less than 0.1% cross-reactivity with neuron-specific enolase. Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. The gene coding for the 48 kDa form of non-neuronal enolase also codes for a shorter 37 kDa isoform, myc-binding protein 1 (MBP-1) lacking the N-terminal 93 amino acids of non-neuronal enolase. |
|
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Native human NNE
- Approx. Protein Concentrations
- IgG concentration 5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Frozen | |
1/25 | 1/100 |
| Immunohistology - Paraffin | |
1/25 | 1/100 |
| Western Blotting | |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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References for Non-Neuronal Enolase antibody
-
Wang, W. et al. (2005) Identification of alpha-enolase as a nuclear DNA-binding protein in the zona fasciculata but not the zona reticularis of the human adrenal cortex.
J Endocrinol. 184: 85-94. -
Prunotto, M. et al. (2010) Endocellular polyamine availability modulates epithelial-to-mesenchymal transition and unfolded protein response in MDCK cells.
Lab Invest. 90: 929-39. -
Kimura Y et al. (2016) Circulating antibodies to α-enolase and phospholipase A2 receptor and composition of glomerular deposits in Japanese patients with primary or secondary membranous nephropathy.
Clin Exp Nephrol. Feb 1. [Epub ahead of print] -
Garcia, A. et al. (2014) Immunohistochemical evaluation of the goat forestomach during prenatal development.
J Vet Sci. 15 (1): 35-43.
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