Non-Neuronal Enolase antibody
Rabbit anti Human non-neuronal enolase polyclonal antibody recognises human non-neuronal enolase, also known as alpha enolase or enolase 1. Rabbit anti non-neuronal enolase demostrates less than 0.1% cross-reactivity with neuron-specific enolase.
Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. The gene coding for the 48 kDa form of non-neuronal enolase also codes for a shorter 37 kDa isoform, myc-binding protein 1 (MBP-1) lacking the N-terminal 93 amino acids of non-neuronal enolase.
Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. The gene coding for the 48 kDa form of non-neuronal enolase also codes for a shorter 37 kDa isoform, myc-binding protein 1 (MBP-1) lacking the N-terminal 93 amino acids of non-neuronal enolase.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Native human NNE
- Approx. Protein Concentrations
- IgG concentration 5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P06733 Related reagents
- Entrez Gene
- ENO1 Related reagents
- Regulatory
- For research purposes only
Applications of Non-Neuronal Enolase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Frozen | 1/25 | 1/100 | |
| Immunohistology - Paraffin | 1/25 | 1/100 | |
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
References for Non-Neuronal Enolase antibody
-
Wang, W. et al. (2005) Identification of alpha-enolase as a nuclear DNA-binding protein in the zona fasciculata but not the zona reticularis of the human adrenal cortex.
J Endocrinol. 184: 85-94. -
Prunotto, M. et al. (2010) Endocellular polyamine availability modulates epithelial-to-mesenchymal transition and unfolded protein response in MDCK cells.
Lab Invest. 90: 929-39. -
Kimura Y et al. (2016) Circulating antibodies to α-enolase and phospholipase A2 receptor and composition of glomerular deposits in Japanese patients with primary or secondary membranous nephropathy.
Clin Exp Nephrol. Feb 1. [Epub ahead of print] -
Garcia, A. et al. (2014) Immunohistochemical evaluation of the goat forestomach during prenatal development.
J Vet Sci. 15 (1): 35-43.
