Rabbit anti Human non-neuronal enolase polyclonal antibody recognises human non-neuronal enolase, also known as alpha enolase or enolase 1. Rabbit anti non-neuronal enolase demostrates less than 0.1% cross-reactivity with neuron-specific enolase.
Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. The gene coding for the 48 kDa form of non-neuronal enolase also codes for a shorter 37 kDa isoform, myc-binding protein 1 (MBP-1) lacking the N-terminal 93 amino acids of non-neuronal enolase.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Native human NNE
- Approx. Protein Concentrations
- IgG concentration 5mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Non-Neuronal Enolase antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Immunohistology - Paraffin
Product Specific References
References for Non-Neuronal Enolase antibody
Wang, W. et al. (2005) Identification of alpha-enolase as a nuclear DNA-binding protein in the zona fasciculata but not the zona reticularis of the human adrenal cortex.
J Endocrinol. 184: 85-94.
Prunotto, M. et al. (2010) Endocellular polyamine availability modulates epithelial-to-mesenchymal transition and unfolded protein response in MDCK cells.
Lab Invest. 90: 929-39.
Kimura Y et al. (2016) Circulating antibodies to α-enolase and phospholipase A2 receptor and composition of glomerular deposits in Japanese patients with primary or secondary membranous nephropathy.
Clin Exp Nephrol. Feb 1. [Epub ahead of print]
Garcia, A. et al. (2014) Immunohistochemical evaluation of the goat forestomach during prenatal development.
J Vet Sci. 15 (1): 35-43.
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