NKX2.5 antibody

Rabbit anti Human NKX2.5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
NKX2.5

Product Code Applications Pack Size List Price Your Price Qty
AHP3088
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SDS Safety Datasheet SDS
WB 0.1 ml loader
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Rabbit anti Human NKX2.5 antibody recognizes homeobox protein NKX-2.5.

Homeobox protein NKX-2.5 is a protein encoded by the NKX2.5 gene. Homeobox-containing genes such as NKX2.5 encode for numerous transcription factors that go on to aid in the development of many structures including the heart (Bartlett et al. 2010). NKX2.5 expression is crucial for the development of atrial, ventricular, and conotruncal septation as well as the atrioventricular (AV) valve and the maintenance of AV conduction. Mutations in the NKX2.5 gene have been linked to congenital heart disease (CHD) (Wang et al. 2017).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human NKX2.5 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide within the N-terminal region of human NKX2.5
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P52952
Entrez Gene
NKX2-5
GO Terms
GO:0001570 vasculogenesis
GO:0001947 heart looping
GO:0003285 septum secundum development
GO:0003682 chromatin binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005667 transcription factor complex
GO:0007512 adult heart development
GO:0008134 transcription factor binding
GO:0008284 positive regulation of cell proliferation
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010667 negative regulation of cardiac muscle cell apoptosis
GO:0010735 positive regulation of transcription via serum response element binding
GO:0010736 serum response element binding
GO:0010765 positive regulation of sodium ion transport
GO:0010832 negative regulation of myotube differentiation
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030097 hemopoiesis
GO:0030878 thyroid gland development
GO:0043586 tongue development
GO:0045666 positive regulation of neuron differentiation
GO:0045823 positive regulation of heart contraction
GO:0045885 positive regulation of survival gene product expression
GO:0046982 protein heterodimerization activity
GO:0048536 spleen development
GO:0051891 positive regulation of cardioblast differentiation
GO:0051929 positive regulation of calcium ion transport via voltage-gated calcium channel activity
GO:0055014 atrial cardiac muscle cell development
GO:0055117 regulation of cardiac muscle contraction
GO:0060037 pharyngeal system development
GO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter
GO:0060412 ventricular septum morphogenesis
GO:0060413 atrial septum morphogenesis
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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