Mono-Methyl-Histone H3 (Lys79) antibody

Rabbit anti Mono-Methyl-Histone H3 (Lys79)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Mono-Methyl-Histone H3 (Lys79)

Product Code Applications Pack Size List Price Your Price Qty
AHP3063
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
Ch WB 0.1 mg loader
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loader
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Rabbit anti Human mono-methyl-histone H3 (Lys79) antibody recognizes histone H3 when mono-methylated at lysine 79.

Histone H3 is one of the four core histones that make up the nucleosome core particle. Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones can be mono-, di- or tri-methylated by histone methyltransferases. Depending on which amino acid residues are methylated, methylation of histones may increase or decrease the transcription of genes. Methylation events that weaken the binding between histone tails and DNA lead to increased transcription because they make the DNA more accessible to transcription factor proteins and RNA polymerase. Methylation of histones is therefore crucial for the regulation of gene expression (Gupta et al. 2010). Lysine 79 of histone H3 may be mono-, di- or tri-methylated by the methylase DOT1. Methylation of lysine 79 of histone H3 acts as a marker of inactive chromatin regions that is essential for silencing of transcription of proteins such as SIR3 (Ng et al. 2003).

Wide species cross-reactivity is expected from Rabbit anti Human mono-methyl-histone H3 (Lys79) antibody based on sequence.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Broad Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to mono-methyl-histone H3 (Lys79) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide including mono-methylated lysine 79 of histone H3
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation 10 µg per ChIP
Western Blotting 1 µg/ml 2 µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P68431
Entrez Gene
HIST1H3F
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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