Mcl-1 antibody
Rabbit anti Human Mcl-1
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Mcl-1
Antibody Quality and Performance Guarantee
| Rabbit anti Human Mcl-1 antibody recognizes an epitope within the central region (IN) of human Myeloid cell leukaemia sequence 1 (MCL-1), an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumours. The MCL-1 protein, localised to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S). MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains. Interaction between these two antagonistic variants can act to regulate cell survival. Rabbit anti Human Mcl-1 antibody (AHP998) recognizes both the MCL-1L (~38 kDa) and MCL-1S (~30 kDa) isoforms of MCL-1. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human MCL-1 (IN) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Immunogen
- A 16 amino acid peptide located near the centre of human MCL-1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
0.5 | 1.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP998 detects a band of approximately 30kDa and 38kDa in Human Raji cell lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
Further Reading
-
Kozopas, K.M. et al. (1993) MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2.
Proc Natl Acad Sci U S A. 90 (8): 3516-20. -
Bae, J. et al. (2000) MCL-1S, a splicing variant of the antiapoptotic BCL-2 family member MCL-1, encodes a proapoptotic protein possessing only the BH3 domain.
J Biol Chem. 275 (33): 25255-61. -
Rinkenberger, J.L. et al. (2000) Mcl-1 deficiency results in peri-implantation embryonic lethality.
Genes Dev. 14 (1): 23-7. -
Michels, J. et al. (2005) Mcl-1.
Int J Biochem Cell Biol. 37 (2): 267-71.
- Synonyms
- Myeloid Cell Leukaemia Sequence 1
- RRID
- AB_2144116
- UniProt
- Q07820
- Entrez Gene
- MCL1
AHP998
147074
155710
163249
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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