IRF7 antibody

Rabbit anti IRF7 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IRF7
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1180
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
List Price Your Price
loader
AHP1180T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti IRF7 antibody detects an epitope within the C-terminal (CT) of human interferon regulatory factor 7 (IRF7), a ~54 kDa transcriptional activator of type I interferons (IFNalpha/beta) in response to local and systemic viral infection. IRF7 is mostly expressed by plasmacytoid dendritic cells (pDC), which differentiate in response to IFNs and virus infection. This then stabilizes the IRF7 protein, in turn leading to increased IFN production.

Multiple IRF7 transcript variants have been identified.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human IRF7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.02%Sodium Azide
Pack Size: 50 µg
<0.1% Sodium Azide (NaN3)
Immunogen
14 amino acid peptide sequence near the carboxy terminus of human IRF7.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 Pack Size: 0.1 mg
1.0 Pack Size: 50 µg
1.0ug/ml Pack Size: 0.1 mg
2.0ug/ml Pack Size: 50 µg
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1180 detects a band of approximately 51kDa in 293 whole cell lysate.

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References for IRF7 antibody

  1. Rautela, J. et al. (2015) Loss of Host Type-I IFN Signaling Accelerates Metastasis and Impairs NK-cell Antitumor Function in Multiple Models of Breast Cancer.
    Cancer Immunol Res. 3 (11): 1207-17.
  2. Saitoh, S.I. et al. (2017) TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells.
    Nat Commun. 8 (1): 1592.

Further Reading

  1. Ning, S. et al. (2005) Regulation of the transcriptional activity of the IRF7 promoter by a pathway independent of interferon signaling.
    J Biol Chem. 280 (13): 12262-70.
  2. Prakash, A. & Levy, D.E. (2006) Regulation of IRF7 through cell type-specific protein stability.
    Biochem Biophys Res Commun. 342 (1): 50-6.

Synonyms
Interferon Regulatory Factor 7
RRID
AB_844627
UniProt
Q92985
Entrez Gene
IRF7
GO Terms
GO:0005886 plasma membrane
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0008063 Toll signaling pathway
GO:0010008 endosome membrane
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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