IRF7 antibody
Rabbit anti IRF7 antibody (AHP1180) used for the evaluation of IRF7 expression on murine cell lysates by western blotting.
Image caption:
Impaired IFN-α production in Itgal-/- BM-pDCs
(c) Shown is immunostaining of the indicated signaling molecules in whole cell lysates from WT, Itgal-/-, and Myd88-/- BM-pDCs.
From: Saitoh SI, Abe F, Kanno A, Tanimura N, Mori Saitoh Y, Fukui R, Shibata T, Sato K, Ichinohe T, Hayashi M, Kubota K, Kozuka-Hata H, Oyama M, Kikko Y, Katada T, Kontani K, Miyake K.
TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells.
Nat Commun. 2017 Nov 17;8(1):1592.
Rabbit anti IRF7 antibody (AHP1180) used for the evaluation of IRF7 expression on murine cell lysates by western blotting.
Image caption:
TLR7 responses and colocalization of TLR7 and Lamp2 in Arl8bGt/Gt and Plekhm2-/- BM-pDCs.
(e) Immunostaining of the indicated signaling molecules in whole cell lysates from WT, Arl8bGt/Gt, and Plekhm2-/- BM-pDCs is shown.
From: Saitoh SI, Abe F, Kanno A, Tanimura N, Mori Saitoh Y, Fukui R, Shibata T, Sato K, Ichinohe T, Hayashi M, Kubota K, Kozuka-Hata H, Oyama M, Kikko Y, Katada T, Kontani K, Miyake K.
TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells.
Nat Commun. 2017 Nov 17;8(1):1592.
Rabbit anti IRF7 (C-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IRF7
- Region
- (C-TERMINAL)
Antibody Quality and Performance Guarantee
| Rabbit anti IRF7 antibody detects an epitope within the C-terminal (CT) of human interferon regulatory factor 7 (IRF7), a ~54 kDa transcriptional activator of type I interferons (IFNalpha/beta) in response to local and systemic viral infection. IRF7 is mostly expressed by plasmacytoid dendritic cells (pDC), which differentiate in response to IFNs and virus infection. This then stabilizes the IRF7 protein, in turn leading to increased IFN production. Multiple IRF7 transcript variants have been identified. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human IRF7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- AHP1180T: <0.1% Sodium Azide (NaN3)
-
AHP1180:
0.02% Sodium Azide - Immunogen
- 14 amino acid peptide sequence near the carboxy terminus of human IRF7.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1.0 Pack Size: 50 µg 0.5 Pack Size: 0.1 mg |
2.0ug/ml Pack Size: 50 µg 1.0ug/ml Pack Size: 0.1 mg |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1180 detects a band of approximately 51kDa in 293 whole cell lysate.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for IRF7 antibody
-
Rautela, J. et al. (2015) Loss of Host Type-I IFN Signaling Accelerates Metastasis and Impairs NK-cell Antitumor Function in Multiple Models of Breast Cancer.
Cancer Immunol Res. 3 (11): 1207-17. -
Saitoh, S.I. et al. (2017) TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells.
Nat Commun. 8 (1): 1592.
Further Reading
-
Ning, S. et al. (2005) Regulation of the transcriptional activity of the IRF7 promoter by a pathway independent of interferon signaling.
J Biol Chem. 280 (13): 12262-70. -
Prakash, A. & Levy, D.E. (2006) Regulation of IRF7 through cell type-specific protein stability.
Biochem Biophys Res Commun. 342 (1): 50-6.
- Synonyms
- Interferon Regulatory Factor 7
- RRID
- AB_844627
- UniProt
- Q92985
- Entrez Gene
- IRF7
- GO Terms
- GO:0005886 plasma membrane
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003704 specific RNA polymerase II transcription factor activity
- GO:0009615 response to virus
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- View More GO Terms
- GO:0008063 Toll signaling pathway
- GO:0010008 endosome membrane
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up