IL-33 antibody
Rabbit anti Human Interleukin-33
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-33
Antibody Quality and Performance Guarantee
| Rabbit anti Human Interleukin-33 antibody recognizes human IL-33 (interleukin-33), formerly known as NF-HEV (nuclear factor from high endothelial venules), a recently described member of the IL-1 superfamily which signals through interaction with the ST2 and Toll-interleukin 1 (IL-1) receptors, and is known to act as an immunoregulator of T helper 2 (Th2) cell cytokine synthesis. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antiserum to human IL-33 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-33
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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- Synonyms
- NF-HEV
- RRID
- AB_2124144
- UniProt
- O95760
- Entrez Gene
- IL33
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0043032 positive regulation of macrophage activation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0050729 positive regulation of inflammatory response
- GO:0090197 positive regulation of chemokine secretion
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