IL-15 antibody

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Rabbit anti Human Interleukin-15

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP300G E FN IP P* WB 0.1 mg
Rabbit anti Human Interleukin-15 antibody recognizes human IL-15 (Interleukin-15), a 14kDa immunomodulatory glycoprotein expressed by multiple tissues and cell types, which primarily stimulates T lymphocytes, but is also critical for the development and activation of natural killer cells (NK).

IL-15 shares functional properties with IL-2 and binds with high affinity to the IL-15 receptor (IL-15R), consisting of the IL-15R alpha chain, and also the IL-2Rbeta and common gamma chains used by the IL-2 receptor. Affects of IL-15 have been linked with cells and tissues unrelated to the immune system, and increased expression of IL-15 has been demonstrated in Rheumatoid Arthritis (RA), ulcerative colitis (UC) Chron’s disease (CD) and lymphoid malignancies, and also conditions affiliated with HIV and HTLV-1 infection.

Rabbit anti Human Interleukin-15 antibody will neutralise IL-15 activity in bioassay systems.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to human IL-15 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human IL-15
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage the addition of 0.09% Sodium Azide is recommended.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P40933 Related reagents
Entrez Gene
IL15 Related reagents
GO Terms
GO:0004871 signal transducer activity
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005634 nucleus
GO:0005768 endosome
GO:0005794 Golgi apparatus
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0032740 positive regulation of interleukin-17 production
GO:0034105 positive regulation of tissue remodeling
GO:0050729 positive regulation of inflammatory response
Regulatory
For research purposes only

Applications of IL-15 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 0.15 0.2ug/ml
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting 0.1 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
For functional studies do not use Azide.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for IL-15 antibody

  1. Kurys, G. et al. (2000) The long signal peptide isoform and its alternative processing direct the intracellular trafficking of interleukin-15.
    J. Biol. Chem. 275: 30653 - 30659.
  2. Fehniger, T.A. and Caligiuri, M.A. et al (2001) Interleukin 15: biology and relevance to human disease.
    BLOOD. 97: 14-32.