IL-15 antibody
Rabbit anti Human Interleukin-15
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-15
Rabbit anti Human Interleukin-15 antibody recognizes human IL-15 (Interleukin-15), a 14kDa immunomodulatory glycoprotein expressed by multiple tissues and cell types, which primarily stimulates T lymphocytes, but is also critical for the development and activation of natural killer cells (NK). IL-15 shares functional properties with IL-2 and binds with high affinity to the IL-15 receptor (IL-15R), consisting of the IL-15R alpha chain, and also the IL-2Rbeta and common gamma chains used by the IL-2 receptor. Affects of IL-15 have been linked with cells and tissues unrelated to the immune system, and increased expression of IL-15 has been demonstrated in Rheumatoid Arthritis (RA), ulcerative colitis (UC) Chron’s disease (CD) and lymphoid malignancies, and also conditions affiliated with HIV and HTLV-1 infection. Rabbit anti Human Interleukin-15 antibody will neutralise IL-15 activity in bioassay systems. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Antiserum Preparation
- Antisera to human IL-15 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-15.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.5ug/ml | ||
Functional Assays | 0.15 | 0.2ug/ml | |
Immunohistology - Paraffin 1 | |||
Immunoprecipitation | |||
Western Blotting | 0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Instructions For Use
- For functional studies do not use Azide.
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References for IL-15 antibody
-
Kurys, G. et al. (2000) The long signal peptide isoform and its alternative processing direct the intracellular trafficking of interleukin-15.
J. Biol. Chem. 275: 30653 - 30659. -
Fehniger, T.A. and Caligiuri, M.A. et al (2001) Interleukin 15: biology and relevance to human disease.
BLOOD. 97: 14-32.
- RRID
- AB_2124584
- UniProt
- P40933
- Entrez Gene
- IL15
- GO Terms
- GO:0004871 signal transducer activity
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005126 cytokine receptor binding
- GO:0005615 extracellular space
- GO:0005624 membrane fraction
- GO:0005634 nucleus
- GO:0005768 endosome
- GO:0005794 Golgi apparatus
- View More GO Terms
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0032740 positive regulation of interleukin-17 production
- GO:0034105 positive regulation of tissue remodeling
- GO:0050729 positive regulation of inflammatory response
AHP300G
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