IgM antibody

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Goat F(ab')2 anti Human IgM:FITC

Goat F(ab')2 anti Human IgM

Goat F(ab')2 anti Human IgM:RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR146F
Datasheet
C F IF
MSDS
0.5 mg loader

STAR146
Datasheet
E
MSDS
0.5 mg loader

STAR146PE
Datasheet
F
MSDS
0.5 ml loader

Goat F(ab')2 anti Human IgM antibody recognizes the heavy chain of human IgM and has been cross absorbed against human IgA and IgG. Goat F(ab')2 anti Human IgM antibody might cross react with IgM from other species.

Product Details

Target Species
Human
Product Form
F(ab')2 fragments of purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
F(ab')2 fragments of purified IgG - liquid
Product Form
F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - liquid
Antiserum Preparation
Antisera to human IgM were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
F(ab')2 fragments were prepared by pepsin digestion.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
None present
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Sucrose
Immunogen
Human IgM.
Approx. Protein Concentrations
F(ab')2 concentration 0.5 mg/ml
Approx. Protein Concentrations
F(ab')2 concentration 0.5 mg/ml
Approx. Protein Concentrations
F(ab')2 concentration 0.25 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
6 months from date of despatch

More Information

UniProt
P01871
Entrez Gene
IGHM
GO Terms
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005624 membrane fraction
Regulatory
For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunofluorescence Neat 1/10
Immunohistology - Frozen
ELISA
Flow Cytometry 1/20 1/30
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Product Specific References

References for IgM antibody

  1. Mpakou, V.E. et al. (2017) Quantitative and qualitative analysis of regulatory T cells in B cell chronic lymphocytic leukemia.
    Leuk Res. 60: 74-81.
  2. Lu, D.R. et al. (2014) Identifying functional anti-Staphylococcus aureus antibodies by sequencing antibody repertoires of patient plasmablasts.
    Clin Immunol. 152 (1-2): 77-89.