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IgG antibody

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Anti Human IgG F(ab')2 Antibody thumbnail image 1 Anti Human IgG F(ab')2 Antibody thumbnail image 2
Anti Human IgG F(ab')2 Antibody gallery image 1
Inhibition of Adalimumab binding to TNFα by HCA202. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab (HCA202) titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using Goat anti-Human Fab antibody (STAR126P).
Anti Human IgG F(ab')2 Antibody gallery image 2
Published customer image:
Horseradish peroxidase conjugated Goat anti Human IgG F(ab')2 antibody (STAR126P) used to detect bound human antibodies to GFP in ELISA
Image caption:
Effect of long-term TCEP incubation on antgen binding Titration ELISA of 4 different anti‐GFP Fabs: AbD50086ad, AbD50508ad, AbD51109ad, AbD51340ad, after 1 week of incubation at 4°C with or without 5mM TCEP. mGFP was coated, anti‐GFP Fabs were titrated and detection was performed with anti‐Fab‐HRP. Error bars represent standard deviation of 2 measurements. Logistic fit was applied.

From: Hentrich C, Putyrski M, Hanuschka H, Preis W, Kellmann SJ, Wich M, Cavada M, Hanselka S, Lelyveld VS, Ylera F.
Engineered reversible inhibition of SpyCatcher reactivity enables rapid generation of bispecific antibodies.
Nat Commun. 2024 Jul 15;15(1):5939.
doi: 10.1038/s41467-024-50296-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti Human IgG F(ab')2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
F(ab')2

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Product Code Applications Pack Size List Price Your Price Qty
STAR126
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
List Price Your Price
loader

Goat anti Human IgG F(ab')2 antibody recognizes Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity.

Goat anti Human IgG F(ab')2 antibody demonstrates minimal cross reactivity with bovine, horse and mouse IgG; other species have not been tested.
No reactivity is seen with the Fc portion of human IgG or non-immunoglobulin serum proteins.

This antibody is a performance tested, matched reagent for use with Bio-Rad HuCAL antibodies.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human IgG F(ab')2
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
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References for IgG antibody

  1. Eriksson, O. et al. (2011) Development of an antibody to bovine IL-2 reveals multifunctional CD4 T(EM) cells in cattle naturally infected with bovine tuberculosis.
    PLoS One. 6 (12): e29194.
  2. Back, J.W. et al. (2012) Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.
    Protein Eng Des Sel. 25: 251-9.
  3. Eriksson, O. et al. (2018) Pancreatic imaging using an antibody fragment targeting the zinc transporter type 8: a direct comparison with radio-iodinated Exendin-4.
    Acta Diabetol. 55 (1): 49-57.
  4. Wege, A.K. et al. (2020) A novel rabbit derived anti-HER2 antibody with pronounced therapeutic effectiveness on HER2-positive breast cancer cells in vitro and in humanized tumor mice (HTM).
    J Transl Med. 18 (1): 316.
  5. Suskiewicz, M.J. et al. (2019) Structure of McsB, a protein kinase for regulated arginine phosphorylation.
    Nat Chem Biol. 15 (5): 510-518.
  6. Hentrich, C. et al. (2024) Engineered reversible inhibition of SpyCatcher reactivity enables rapid generation of bispecific antibodies.
    Nat Commun. 15 (1): 5939.

Flow Cytometry

RRID
AB_1102648
UniProt
P01834
P0CG04
Entrez Gene
IGKC
IGLV2-14
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response

STAR126

152214 154892 159579 167364

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