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IgG antibody

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Anti Human IgG F(ab')2 Antibody gallery image 1
Inhibition of Adalimumab binding to TNFα by HCA202. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab (HCA202) titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using Goat anti-Human Fab antibody (STAR126P).

Goat anti Human IgG F(ab')2:Alk. Phos

Goat anti Human IgG F(ab')2:Biotin

Goat anti Human IgG F(ab')2:FITC

Goat anti Human IgG F(ab')2:HRP

Goat anti Human IgG F(ab')2

Goat anti Human IgG F(ab')2:TRITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR126A
Datasheet
E WB
SDS
0.5 ml loader

STAR126B
Datasheet
C E F WB
SDS
0.5 mg loader

STAR126F
Datasheet
C F IF
SDS
0.5 mg loader

STAR126P
Datasheet
E WB
SDS
0.5 ml loader

STAR126
Datasheet
E
SDS
0.5 mg loader

STAR126TRC
Datasheet
C F IF
SDS
0.5 mg loader

Goat anti Human IgG F(ab')2 antibody recognizes Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity.

Goat anti Human IgG F(ab')2 antibody demonstrates minimal cross reactivity with bovine, horse and mouse IgG; other species have not been tested.
No reactivity is seen with the Fc portion of human IgG or non-immunoglobulin serum proteins.

This antibody is a performance tested, matched reagent for use with Bio-Rad HuCAL antibodies.

Product Details

Target Species
Human
Product Form
Purified Ig conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified Ig prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography.
Preparation
Purified IgG prepared by affinity chromatography.
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography.
Preparation
Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
1.5% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1.5% Bovine Serum Albumin.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1.5% Bovine Serum Albumin.
Preservative Stabilisers
0.01% Thiomersal
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
1.5% Bovine Serum Albumin.
Immunogen
Human IgG F(ab')2
Approx. Protein Concentrations
IgG concentration 0.6mg/ml
Approx. Protein Concentrations
1.0 mg/ml
Approx. Protein Concentrations
1.0 mg/ml
Approx. Protein Concentrations
0.5 mg/ml
Approx. Protein Concentrations
1.0 mg/ml
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01834
P0CG04
Entrez Gene
IGKC
IGLV2-14
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Western Blotting 1/1000 1/10000
ELISA 1/1,000 1/10,000
Flow Cytometry 1/200 1/1000
Immunohistology - Frozen 1/200 1/1000
Western Blotting 1/1,000 1/10,000
Flow Cytometry 1/50 1/200
Immunofluorescence 1/50 1/200
Immunohistology - Frozen 1/50 1/200
ELISA 1/1000 1/10000
Western Blotting 1/1000 1/10000
ELISA
Flow Cytometry 1/50 1/200
Immunofluorescence 1/50 1/200
Immunohistology - Frozen 1/50 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Product Specific References

References for IgG antibody

  1. Eriksson, O. et al. (2011) Development of an antibody to bovine IL-2 reveals multifunctional CD4 T(EM) cells in cattle naturally infected with bovine tuberculosis.
    PLoS One. 6 (12): e29194.
  2. Back, J.W. et al. (2012) Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.
    Protein Eng Des Sel. 25: 251-9.
  3. Eriksson, O. et al. (2017) Pancreatic imaging using an antibody fragment targeting the zinc transporter type 8: a direct comparison with radio-iodinated Exendin-4.
    Acta Diabetol. Oct 24 [Epub ahead of print].
  4. Wege, A.K. et al. (2020) A novel rabbit derived anti-HER2 antibody with pronounced therapeutic effectiveness on HER2-positive breast cancer cells in vitro and in humanized tumor mice (HTM).
    J Transl Med. 18 (1): 316.

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