IgG antibody

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Anti Human IgG F(ab')2 Antibody gallery image 1 — Inhibition of Adalimumab binding to TNFα by **HCA202**. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab (**HCA202**) titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using Goat anti-Human Fab antibody (STAR126P).

Goat anti Human IgG F(ab')2:Alk. Phos

Goat anti Human IgG F(ab')2:Biotin

Goat anti Human IgG F(ab')2:FITC

Goat anti Human IgG F(ab')2:HRP

Goat anti Human IgG F(ab')2

Goat anti Human IgG F(ab')2:TRITC

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size
STAR126A	E WB	0.5 ml
STAR126B	C E F WB	0.5 mg
STAR126F	C F IF	0.5 mg
STAR126P	E WB	0.5 ml
STAR126	E	0.5 mg
STAR126TRC	C F IF	0.5 mg

Goat anti Human IgG F(ab')2 antibody recognizes Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity.

Goat anti Human IgG F(ab')2 antibody demonstrates minimal cross reactivity with bovine, horse and mouse IgG; other species have not been tested.
No reactivity is seen with the Fc portion of human IgG or non-immunoglobulin serum proteins.

This antibody is a performance tested, matched reagent for use with Bio-Rad HuCAL antibodies.

Product Details

Target Species: Human
Product Form: Purified Ig conjugated to Alkaline Phosphatase - liquid
Product Form: Purified IgG conjugated to Biotin - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Purified Ig prepared by affinity chromatography
Preparation: Purified IgG prepared by affinity chromatography.
Preparation: Purified IgG prepared by affinity chromatography.
Preparation: Purified IgG prepared by affinity chromatography
Preparation: Purified IgG prepared by affinity chromatography.
Preparation: Purified IgG prepared by affinity chromatography.
Buffer Solution: TRIS buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.05% Sodium Azide (NaN₃)
1.5% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1.5% Bovine Serum Albumin.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin.
Preservative Stabilisers: 0.01% Thiomersal
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.05% Sodium Azide (NaN₃)
1.5% Bovine Serum Albumin.
Immunogen: Human IgG F(ab')2
Approx. Protein Concentrations: IgG concentration 0.6mg/ml
Approx. Protein Concentrations: 1.0 mg/ml
Approx. Protein Concentrations: 1.0 mg/ml
Approx. Protein Concentrations: 0.5 mg/ml
Approx. Protein Concentrations: 1.0 mg/ml
Approx. Protein Concentrations: 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01834 Related reagents; P0CG04 Related reagents
Entrez Gene: IGKC Related reagents; IGLV2-14 Related reagents
GO Terms: GO:0005515 protein binding; GO:0003823 antigen binding; GO:0005576 extracellular region; GO:0006958 complement activation, classical pathway; GO:0045087 innate immune response
Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/1000	1/10000
Western Blotting	1/1000	1/10000
ELISA	1/1,000	1/10,000
Flow Cytometry	1/200	1/1000
Immunohistology - Frozen	1/200	1/1000
Western Blotting	1/1,000	1/10,000
Flow Cytometry	1/50	1/200
Immunofluorescence	1/50	1/200
Immunohistology - Frozen	1/50	1/200
ELISA	1/1000	1/10000
Western Blotting	1/1000	1/10000
ELISA
Flow Cytometry	1/50	1/200
Immunofluorescence	1/50	1/200
Immunohistology - Frozen	1/50	1/200

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
AbGUARD® HRP Stabilizer Plus	BUF052A	100 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052B	500 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052C	1000 ml	C E P WB
TMB Signal+	BUF054A	100 ml	E
TMB Core	BUF056A	100 ml	E
TMB Core+	BUF062A	100 ml	E
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Product Specific References

References for IgG antibody

Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS One. 6: e29194.
Back, J.W. et al. (2012) Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.
Protein Eng Des Sel. 25: 251-9.
Eriksson, O. et al. (2017) Pancreatic imaging using an antibody fragment targeting the zinc transporter type 8: a direct comparison with radio-iodinated Exendin-4.
Acta Diabetol. Oct 24 [Epub ahead of print].

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