IgG antibody

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Goat anti Human IgG Gamma Chain:HRP

Goat anti Human IgG Gamma Chain

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR106P C E 0.8 mg
STAR106 C E 0.8 mg
Goat anti Human IgG gamma chain antibody recognizes human IgG and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses. Goat anti Human IgG gamma Chain antibody recognizes the Fc region of IgG, and has been adsorbed against mouse immunoglobulins to reduce species cross-reactivity.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
HRP stabiliser (BUF052A)
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human IgG.
Approx. Protein Concentrations
IgG concentration 0.8 mg/ml
Approx. Protein Concentrations
IgG concentration 0.8 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
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GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/3000
Immunohistology - Frozen
ELISA 1ug/ml 5ug/ml
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

Western Blotting

Product Specific References

References for IgG antibody

  1. Nielsen, L.S. et al. (2010) Single-batch production of recombinant human polyclonal antibodies.
    Mol Biotechnol. 45 (3): 257-66.
  2. Nordström, T. et al. (2011) Human Siglec-5 inhibitory receptor and immunoglobulin A (IgA) have separate binding sites in streptococcal beta protein.
    J Biol Chem. 286 (39): 33981-91.
  3. Hanitsch, L.G. et al. (2015) Late-Onset Disseminated Mycobacterium avium intracellulare Complex Infection (MAC), Cerebral Toxoplasmosis and Salmonella Sepsis in a German Caucasian Patient with Unusual Anti-Interferon-Gamma IgG1 Autoantibodies.
    J Clin Immunol. 35 (4): 361-5.
  4. Zheng, L. et al. (2015) Streptococcal 5′-nucleotidase A (S5nA), a novel group A Streptococcal virulence factor that facilitates immune evasion.
    J Biol Chem. pii: jbc.M115.677443.
  5. Le, Y. et al. (2016) Immunogenicity of Murine mPEG-Red Blood Cells and the Risk of Anti-PEG Antibodies in Human Blood Donors.
    Exp Hematol. Nov 15. pii: S0301-472X(16)30702-0. [Epub ahead of print]
  6. Zheng, L. et al. (2015) Streptococcal 5′-Nucleotidase A (S5nA), a Novel Streptococcus pyogenes Virulence Factor That Facilitates Immune Evasion.
    J Biol Chem. 290 (52): 31126-37.