IgE antibody

Goat anti Human IgE:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgE

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STAR147B
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Goat anti Human IgE polyclonal antibody recognizes human IgE. Minimal cross-reactivity (<0.1%) is expected with other human immunoglobulins and light chains.

Goat anti Human IgE in a biotinylated format has been successfully employed as a detection reagent in the development of a sensitive sandwich ELISA for the measurement of human IgE.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Antiserum Preparation
Antisera to human IgE were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using antigen coupled to agarose beads..
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Native human IgE.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1:10000 1:100000
Western Blotting 1:10000 1:100000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgE antibody

  1. Khan, F.M. et al. (2012) Basophil activation test compared to skin prick test and fluorescence enzyme immunoassay for aeroallergen-specific Immunoglobulin-E.
    Allergy Asthma Clin Immunol. 8 (1): 1.
  2. Pinot de Moira, A. et al. (2013) Effects of treatment on IgE responses against parasite allergen-like proteins and immunity to reinfection in childhood schistosome and hookworm coinfections.
    Infect Immun. 81 (1): 23-32.
  3. Zheng, W. et al. (2016) Upregulated expression of substance P in basophils of the patients with chronic spontaneous urticaria: induction of histamine release and basophil accumulation by substance P.
    Cell Biol Toxicol. 32 (3): 217-28.
  4. Jin Y et al. (2015) Allergenic response to squid (Todarodes pacificus) tropomyosin Tod p1 structure modifications induced by high hydrostatic pressure.
    Food Chem Toxicol. 76: 86-93.
  5. Wei, J.F. et al. (2009) Induction of mast cell accumulation, histamine release and skin edema by N49 phospholipase A2.
    BMC Immunol. 10: 21.

RRID
AB_10672882
UniProt
P01854
Entrez Gene
IGHE
GO Terms
GO:0003823 antigen binding
GO:0006955 immune response

STAR147B

146232 152812 162298

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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