IgA antibody

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Goat anti Human IgA:HRP

Goat anti Human IgA Alpha Chain

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
5104-2404
Datasheet
C E WB
SDS
1 mg loader

5104-2004
Datasheet
C E WB
SDS
1 mg loader

Goat anti Human IgA alpha chain antibody recognizes the alpha chain of human immunoglobulin A.

Goat anti Human IgA alpha chain antibody has been cross-adsorbed to minimise cross-reactivity with other human immunoglobulin subclasses.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
Product Form
Purified Ig - liquid
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified Ig prepared by immunoaffinity isolation on an agarose column
Preparation
Purified Ig prepared by immuno affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Gentamicin Sulphate
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Highly purified human IgA alpha-chain.
Approx. Protein Concentrations
1.0 mg/ml after reconstitution
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01876
P01877
Entrez Gene
IGHA1
IGHA2
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0006955 immune response
GO:0018298 protein-chromophore linkage
GO:0005624 membrane fraction
Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/50000
Immunohistology - Frozen 1/500 1/3000
Western Blotting 1/1000 1/5000
ELISA
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for IgA antibody

  1. Lantto, J. et al. (2011) Capturing the natural diversity of the human antibody response against vaccinia virus.
    J Virol. 85 (4): 1820-33.