IgA antibody
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Goat anti Human IgA alpha chain antibody recognizes the alpha chain of human immunoglobulin A.
Goat anti Human IgA alpha chain antibody has been cross-adsorbed to minimise cross-reactivity with other human immunoglobulin subclasses.
Goat anti Human IgA alpha chain antibody has been cross-adsorbed to minimise cross-reactivity with other human immunoglobulin subclasses.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
- Product Form
- Purified Ig - liquid
- Reconstitution
- Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified Ig prepared by immunoaffinity isolation on an agarose column
- Preparation
- Purified Ig prepared by immuno affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- 0.02M Potassium Phosphate
0.15M Sodium Chloride - Preservative Stabilisers
0.01% Gentamicin Sulphate 1% Bovine Serum Albumin - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Highly purified human IgA alpha-chain.
- Approx. Protein Concentrations
- 1.0 mg/ml after reconstitution
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01876
- P01877
- Entrez Gene
- IGHA1
- IGHA2
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0006955 immune response
- GO:0018298 protein-chromophore linkage
- GO:0005624 membrane fraction
- Regulatory
- For research purposes only
Applications of IgA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/50000 | |
| Immunohistology - Frozen | 1/500 | 1/3000 | |
| Western Blotting | 1/1000 | 1/5000 | |
| ELISA | |||
| Immunohistology - Frozen | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgA antibody
-
Lantto, J. et al. (2011) Capturing the natural diversity of the human antibody response against vaccinia virus.
J Virol. 85 (4): 1820-33. -
Gerretsen, H.E. et al. (2018) Antibodies in lymphocyte supernatants can distinguish between neutralising antibodies induced by RSV vaccination and pre-existing antibodies induced by natural infection.
Vaccine. 36 (46): 6988-94.
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