HRK antibody

Product Code Applications Pack Size List Price Your Price Qty
AHP1178
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
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loader
AHP1178T
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SDS Safety Datasheet SDS
WB 50 µg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti HRK antibody detects Harakiri (HRK), also known as Neuronal death protein DP5 or BH3-interacting domain-containing protein 3. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and haematopoietic cell apoptosis, in particular to growth factor deprivation.

Aberrant methylation of HRK is frequently detected in primary colorectal cancers. Despite its predicted molecular weight of ~18 kDa, HRK often migrates at ~12-15 kDa.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to HRK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.02%Sodium Azide
Pack Size: 50 µg
<0.1% Sodium Azide (NaN3)
Immunogen
15 amino acid peptide from near the centre of human HRK (NP_003797).
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 2 5ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1178 detects a band of approximately 13kDa in mouse pancreas tissue lysate.

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Description Goat anti Rabbit IgG (Fc):Biotin
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Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
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Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
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Description Goat anti Rabbit IgG (H/L):HRP
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Description Sheep anti Rabbit IgG:FITC
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
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Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

References for HRK antibody

  1. Nakamura M. et al. (2005) Frequent HRK inactivation associated with low apoptotic index in secondary glioblastomas.
    Acta Neuropathol (Berl). 110: 402-410.
  2. Sanz, C. et al. (2001) Interleukin 3-dependent activation of DREAM is involved in transcriptional silencing of the apoptotic Hrk gene in hematopoietic progenitor cells.
    EMBO J. 20: 2286-2292.
  3. Rizvi, F. et al. (2011) Mitochondrial dysfunction links ceramide activated HRK expression and cell death.
    PLoS One. 6: e18137.

Further Reading

  1. Sunayama, J. et al. (2004) Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32. .
    Cell Death Differ. 11: 771-781

Western Blotting

Synonyms
DP5
RRID
AB_2248434
UniProt
O00198
Entrez Gene
HRK
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0006917 induction of apoptosis
GO:0008634 negative regulation of survival gene product expression
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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