HRK antibody

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Rabbit anti HRK

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1178T
Datasheet
WB
MSDS
50 µg loader

AHP1178
Datasheet
WB
MSDS
0.1 mg loader

Rabbit anti HRK antibody detects Harakiri (HRK), also known as Neuronal death protein DP5 or BH3-interacting domain-containing protein 3. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and haematopoietic cell apoptosis, in particular to growth factor deprivation.

Aberrant methylation of HRK is frequently detected in primary colorectal cancers. Despite its predicted molecular weight of ~18 kDa, HRK often migrates at ~12-15 kDa.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to HRK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
15 amino acid peptide from near the centre of human HRK (NP_003797).
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O00198
Entrez Gene
HRK
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0006917 induction of apoptosis
GO:0008634 negative regulation of survival gene product expression
Regulatory
For research purposes only

Applications of HRK antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 2 5ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1178 detects a band of approximately 13kDa in mouse pancreas tissue lysate.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Western Blotting

Product Specific References

References for HRK antibody

  1. Nakamura M. et al. (2005) Frequent HRK inactivation associated with low apoptotic index in secondary glioblastomas.
    Acta Neuropathol (Berl). 110: 402-410.
  2. Sanz, C. et al. (2001) Interleukin 3-dependent activation of DREAM is involved in transcriptional silencing of the apoptotic Hrk gene in hematopoietic progenitor cells.
    EMBO J. 20: 2286-2292.
  3. Rizvi, F. et al. (2011) Mitochondrial dysfunction links ceramide activated HRK expression and cell death.
    PLoS One. 6: e18137.

Further Reading

  1. Sunayama, J. et al. (2004) Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32. .
    Cell Death Differ. 11: 771-781