Rabbit anti HRK antibody detects Harakiri (HRK), also known as Neuronal death protein DP5 or BH3-interacting domain-containing protein 3. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and haematopoietic cell apoptosis, in particular to growth factor deprivation.
Aberrant methylation of HRK is frequently detected in primary colorectal cancers. Despite its predicted molecular weight of ~18 kDa, HRK often migrates at ~12-15 kDa.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to HRK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 15 amino acid peptide from near the centre of human HRK (NP_003797).
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
induction of apoptosis
negative regulation of survival gene product expression
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of HRK antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1178 detects a band of approximately 13kDa in mouse pancreas tissue lysate.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for HRK antibody
Nakamura M. et al. (2005) Frequent HRK inactivation associated with low apoptotic index in secondary glioblastomas.
Acta Neuropathol (Berl). 110: 402-410.
Sanz, C. et al. (2001) Interleukin 3-dependent activation of DREAM is involved in transcriptional silencing of the apoptotic Hrk gene in hematopoietic progenitor cells.
EMBO J. 20: 2286-2292.
Rizvi, F. et al. (2011) Mitochondrial dysfunction links ceramide activated HRK expression and cell death.
PLoS One. 6: e18137.
Sunayama, J. et al. (2004) Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32. .
Cell Death Differ. 11: 771-781
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