Histone H4 antibody

Rabbit anti Human Histone H4

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Histone H4

Product Code Applications Pack Size List Price Your Price Qty
AHP3069
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
Ch WB 0.1 ml loader
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Rabbit anti Human histone H4 antibody recognizes histone H4.

Histone H4 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histone H4 is important for the regulation of the structure and function of chromatin. The sequence variants and variable modification states of histone H4 are thought to play a role in both long-term and short-term regulation of genes (Huang et al. 2005) (Taylor et al. 2013).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human histone H4 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide corresponding to amino acids 43- 54 of human histone H4
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation 2 µl per ChIP 10 µl per ChIP
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P62805
Entrez Gene
HIST2H4B
GO Terms
GO:0005515 protein binding
GO:0000723 telomere maintenance
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0005654 nucleoplasm
GO:0048015 phosphatidylinositol-mediated signaling
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0045653 negative regulation of megakaryocyte differentiation
View more products with HISTONE specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products