Histone H2B (Ac15) antibody

Rabbit anti Human Histone H2B (Ac15)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Histone H2B (Ac15)

Product Code Applications Pack Size List Price Your Price Qty
AHP3047
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human histone H2B (Ac15) antibody recognizes histone H2B when acetylated at lysine 15.

Histone H2B is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histone H2B aids the regulation of chromatin structure and function via post-translational modifications of specialized histone variants (Molden et al. 2015). Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions. Acetylation of a specific lysine residues on histones allows binding to bromine-containing domains of particular transcription and chromatin regulatory proteins (Kadiyala et al. 2012).

Wide species cross-reactivity is expected from Rabbit anti Human histone H2B (Ac15) antibody based on sequence.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human histone H2B (Ac15) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide containing acetylated lysine 15 of human histone H2B
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
Q99879
Entrez Gene
HIST1H2BM
GO Terms
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0006334 nucleosome assembly
GO:0005634 nucleus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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