Histone H2A.X (pTyr142) antibody

Rabbit anti Human Histone H2A.X (pTyr142)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Histone H2A.X (pTyr142)

Product Code Applications Pack Size List Price Your Price Qty
AHP3045
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
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loader
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Rabbit anti Human H2A.X (pTyr142) antibody recognizes histone H2A.X when phosphorylated at tyrosine 142.

Histone H2A.X is a member of the histone H2A family, one of the four core histones making up the nucleosome core particle. Histone H2A variant H2A.X is a variant of histone H2A that regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein, to the sites of DNA damage. Phosphorylation of histone H2A.X at tyrosine 142 is implicated in a number of biological processes, including DNA repair. K-Ras-ERK1/2 down-regulates phosphorylation of H2A.X at the site of tyrosine 142 by increasing degradation of WSTF and contributes to the progression of gastric cancer (Dong et al. 2019).

Wide species cross-reactivity is expected from Rabbit anti Human histone H2A.X (pTyr142) antibody based on sequence.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human histone H2A.X (pTyr142) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide containing phosphorylated tyrosine 142 of human histone H2A.X
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P16104
Entrez Gene
H2AFX
GO Terms
GO:0000077 DNA damage checkpoint
GO:0000724 double-strand break repair via homologous recombination
GO:0000786 nucleosome
GO:0006334 nucleosome assembly
GO:0042393 histone binding
GO:0005654 nucleoplasm
GO:0007126 meiosis
GO:0010212 response to ionizing radiation
GO:0019899 enzyme binding
GO:0045739 positive regulation of DNA repair
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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