Histone H2A.X (pTyr142) antibody
Rabbit anti Human Histone H2A.X (pTyr142)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Histone H2A.X (pTyr142)
Antibody Quality and Performance Guarantee
| Rabbit anti Human H2A.X (pTyr142) antibody recognizes histone H2A.X when phosphorylated at tyrosine 142. Histone H2A.X is a member of the histone H2A family, one of the four core histones making up the nucleosome core particle. Histone H2A variant H2A.X is a variant of histone H2A that regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein, to the sites of DNA damage. Phosphorylation of histone H2A.X at tyrosine 142 is implicated in a number of biological processes, including DNA repair. K-Ras-ERK1/2 down-regulates phosphorylation of H2A.X at the site of tyrosine 142 by increasing degradation of WSTF and contributes to the progression of gastric cancer (Dong et al. 2019). Wide species cross-reactivity is expected from Rabbit anti Human histone H2A.X (pTyr142) antibody based on sequence. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human histone H2A.X (pTyr142) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- A peptide containing phosphorylated tyrosine 142 of human histone H2A.X
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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- UniProt
- P16104
- Entrez Gene
- H2AFX
- GO Terms
- GO:0000077 DNA damage checkpoint
- GO:0000724 double-strand break repair via homologous recombination
- GO:0000786 nucleosome
- GO:0006334 nucleosome assembly
- GO:0042393 histone binding
- GO:0005654 nucleoplasm
- GO:0007126 meiosis
- GO:0010212 response to ionizing radiation
- GO:0019899 enzyme binding
- View More GO Terms
- GO:0045739 positive regulation of DNA repair
AHP3045
160740
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