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Histone H2A.J antibody

Rabbit anti Human Histone H2A.J

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Histone H2A.J

Product Code Applications Pack Size List Price Your Price Qty
AHP3044
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IB WB 0.1 ml loader
List Price Your Price
loader

Rabbit anti Human histone H2A.J antibody recognizes histone H2A.J.

Histone H2A.J is a 16 kDa variant of the H2A histone. Histones are nuclear proteins that regulate the nucleosome structure of the chromosomal fibres in eukaryotic cells. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histone H2A.J may be linked to senescence and upregulation of the expression of inflammatory genes. Histone H2A.J may therefore play a role in chronic inflammation and age-related diseases (Takana et al. 2020).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human histone H2A.J was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide comprising amino acids 122-129 of human histone H2A.J
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoblotting 1/1000
Western Blotting 1/500 1/2500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
Q9BTM1
Entrez Gene
H2AFJ
GO Terms
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0006334 nucleosome assembly
GO:0005634 nucleus

AHP3044

160727

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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