Histone H2A.J antibody
Rabbit anti Human Histone H2A.J
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Histone H2A.J
Antibody Quality and Performance Guarantee
| Rabbit anti Human histone H2A.J antibody recognizes histone H2A.J. Histone H2A.J is a 16 kDa variant of the H2A histone. Histones are nuclear proteins that regulate the nucleosome structure of the chromosomal fibres in eukaryotic cells. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histone H2A.J may be linked to senescence and upregulation of the expression of inflammatory genes. Histone H2A.J may therefore play a role in chronic inflammation and age-related diseases (Takana et al. 2020). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human histone H2A.J was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- A peptide comprising amino acids 122-129 of human histone H2A.J
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoblotting |  |
1/1000 | |
| Western Blotting | |
1/500 | 1/2500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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|---|---|---|---|---|---|---|---|
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
- UniProt
- Q9BTM1
- Entrez Gene
- H2AFJ
- GO Terms
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0006334 nucleosome assembly
- GO:0005634 nucleus
AHP3044
160727
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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