Rabbit anti Human heme oxygenase 1 antibody
recognizes human heme oxygenase-1 (HO-1), a 288 amino acid enzyme with a crucial role in heme catabolism, cleaving the heme ring to form biliverdin which is further processed to form bilirubin by a reductase enzyme. HO-1 activity is highest in the spleen where it is involved in the processing of senescent erythrocytes (Delaby et al. 2012
). HO-1 expression can be increased during cellular stress, including heat shock (Ewing et al. 1992
). Baseline levels of expression are high in certain tissues, including spleen and liver. Enriched enzyme preparations are recommended for experimental analysis.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.065% Sodium Azide (NaN3)
- Synthetic peptide with the amino acid sequence HDLSEALKEATKEVHC corresponding to residues 12-25 (+C-NH2 of human heme oxygenase 1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 6 months from date of despatch
- Entrez Gene
- GO Terms
endothelial cell proliferation
wound healing involved in inflammatory response
negative regulation of leukocyte migration
heme oxygenase (decyclizing) activity
signal transducer activity
endoplasmic reticulum membrane
cellular iron ion homeostasis
intracellular protein kinase cascade
positive regulation of vasodilation
smooth muscle hyperplasia
low-density lipoprotein particle clearance
response to nicotine
heme catabolic process
response to hydrogen peroxide
protein homodimerization activity
positive regulation of I-kappaB kinase/NF-kappaB cascade
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
positive regulation of chemokine biosynthetic process
regulation of angiogenesis
positive regulation of smooth muscle cell proliferation
negative regulation of smooth muscle cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Heme Oxygenase 1 antibody
|Immunohistology - Frozen
|Western Blotting 1
- 1This antibody detects a band of approximately 32kDa under reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human spleen
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Secondary Antibodies Available