Heme Oxygenase 1 antibody

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Rabbit anti Human Heme Oxygenase 1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4915-1050 C WB* 50 µg
Rabbit anti Human heme oxygenase 1 antibody recognizes human heme oxygenase-1 (HO-1), a 288 amino acid enzyme with a crucial role in heme catabolism, cleaving the heme ring to form biliverdin which is further processed to form bilirubin by a reductase enzyme. HO-1 activity is highest in the spleen where it is involved in the processing of senescent erythrocytes (Delaby et al. 2012). HO-1 expression can be increased during cellular stress, including heat shock (Ewing et al. 1992). Baseline levels of expression are high in certain tissues, including spleen and liver. Enriched enzyme preparations are recommended for experimental analysis.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Mouse
Rat
Dog
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.065% Sodium Azide (NaN3)
Immunogen
Synthetic peptide with the amino acid sequence HDLSEALKEATKEVHC corresponding to residues 12-25 (+C-NH2 of human heme oxygenase 1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of despatch.

More Information

UniProt
P09601 Related reagents
Entrez Gene
HMOX1 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0001935 endothelial cell proliferation
GO:0002246 wound healing involved in inflammatory response
GO:0002686 negative regulation of leukocyte migration
GO:0004392 heme oxygenase (decyclizing) activity
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006788 heme oxidation
GO:0006879 cellular iron ion homeostasis
GO:0006916 anti-apoptosis
GO:0007243 intracellular protein kinase cascade
GO:0007588 excretion
GO:0008219 cell death
GO:0045909 positive regulation of vasodilation
GO:0014806 smooth muscle hyperplasia
GO:0019899 enzyme binding
GO:0020037 heme binding
GO:0034101 erythrocyte homeostasis
GO:0034383 low-density lipoprotein particle clearance
GO:0035094 response to nicotine
GO:0042167 heme catabolic process
GO:0042542 response to hydrogen peroxide
GO:0042803 protein homodimerization activity
GO:0051260 protein homooligomerization
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045765 regulation of angiogenesis
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0055085 transmembrane transport
Regulatory
For research purposes only

Applications of Heme Oxygenase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/50 1/1000
Western Blotting 1 1/1000
  1. 1This antibody detects a band of approximately 32kDa under reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Human spleen
Human kidney
Porcine arteries

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB