GRO Beta antibody
Rabbit anti Human GRO beta antibody (AHP773) used to demonstrate CXCL2 expression in human brain tissue biopsy sections by immunofluorescence.
Image caption:
Overexpression of proangiogenic factors in human GBM.
(c) Tissue sections of epilepsy (EP) and glioblastoma (GBM) patients were stained for proangiogenic factors like CXCL2 (green), blue: DAPI (nuclei). Scale bars 100 μm. (d) Graph depicts calculation of the stained area of CXCL2 (d). * p <0.05, ** p <0.01, ns = not significant. Student's t-test. n = 4 in EP and n = 8 in GBM.
From: Urbantat, R.M. Blank, A. Kremenetskaia, I. Vajkoczy, P. Acker, G. Brandenburg, S.
The CXCL2/IL8/CXCR2 Pathway Is Relevant for Brain Tumor Malignancy and Endothelial Cell Function.
Int J Mol Sci. 2021, 22, 2634.
doi: 10.3390/ijms22052634
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Human GRO Beta
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- GRO Beta
Antibody Quality and Performance Guarantee
| Rabbit anti Human GRO beta antibody recognizes human GRO-beta, also known as Growth-regulated protein beta, C-X-C motif chemokine 2, Macrophage inflammatory protein 2-alpha, MIP-2α or CXCL2. GRO beta is a ~7.9 kDa CXC chemokine and one of three closely related, but distinct GRO gene products, which act as chemoattractants and activators of neutrophils and basophils. Like GRO-alpha (CXCL1) and GRO-gamma (CXCL3), GRO-beta contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors, GRO-beta has a lower potency and receptor binding affinity (Geiser et al. 1993). |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not azide.
- Antiserum Preparation
- Antisera to human GRO-beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human GRO-beta.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5ug/ml | |
| Functional Assays | |
0.5 | 1.0ug/ml |
| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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References for GRO Beta antibody
-
Kogan-sakin, I. et al. (2009) Prostate stromal cells produce CXCL-1, CXCL-2, CXCL-3 and IL-8 in response to epithelia-secreted IL-1.
Carcinogenesis. 30 (4): 698-705. -
Urbantat, R.M. et al. (2021) The CXCL2/IL8/CXCR2 Pathway Is Relevant for Brain Tumor Malignancy and Endothelial Cell Function.
Int J Mol Sci. 22 (5): 2634.
Further Reading
-
Haskill, S. et al. (1990) Identification of three related human GRO genes encoding cytokine functions.
Proc Natl Acad Sci U S A. 87 (19): 7732-6. -
Geiser, T. et al. (1993) The interleukin-8-related chemotactic cytokines GRO alpha, GRO beta, and GRO gamma activate human neutrophil and basophil leukocytes.
J Biol Chem. 268 (21): 15419-24.
- Synonyms
- CXCL2
- RRID
- AB_2086316
- UniProt
- P19875
- Entrez Gene
- CXCL2
- GO Terms
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008009 chemokine activity
AHP773
148365
150439
165832
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