FAP Alpha antibody

100% Secure


Rabbit anti Human FAP Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1322
Datasheet
P*
SDS
50 µg loader

Rabbit anti Human FAP alpha antibody detects fibroblast activation protein alpha (FAP alpha), a type II membrane protein belonging to the serine protease family. FAP alpha is upregulated on activated fibroblasts in response to wounding, and may also be involved in epithelial-mesenchymal interactions during development and tissue repair.

Active FAP alpha is a ~170 kDa homodimeric protein which can also exist as a soluble form. FAP alpha can also form heterodimeric complexes with CD26 (DPP4), and these proteins may function co-ordinately to modulate the growth, differentiation, adhesion and metastasis of tumour cells.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to FAP alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide: extracellular domain of human FAP alpha, conjugated to keyhole limpet hemocyanin.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q12884
Entrez Gene
FAP
GO Terms
GO:0004252 serine-type endopeptidase activity
GO:0008239 dipeptidyl-peptidase activity
GO:0004222 metalloendopeptidase activity
GO:0016021 integral to membrane
GO:0006508 proteolysis
GO:0010716 negative regulation of extracellular matrix disassembly
GO:0030054 cell junction
GO:0031258 lamellipodium membrane
GO:0042803 protein homodimerization activity
GO:0043542 endothelial cell migration
Regulatory
For research purposes only

Applications of FAP Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Uterus

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
Histar Detection System STAR3000A C P 50 Tests

Product Specific References

Further Reading

  1. Kelly, T. (2005) Fibroblast activation protein-alpha and dipeptidyl peptidase IV (CD26): cell-surface proteases that activate cell signaling and are potential targets for cancer therapy.
    Drug Resist Updat. 8 (1-2): 51-8.