FAP Alpha antibody

Rabbit anti Human FAP Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
FAP Alpha

Product Code Applications Pack Size List Price Your Price Qty
AHP1322
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Rabbit anti Human FAP alpha antibody detects fibroblast activation protein alpha (FAP alpha), a type II membrane protein belonging to the serine protease family. FAP alpha is upregulated on activated fibroblasts in response to wounding, and may also be involved in epithelial-mesenchymal interactions during development and tissue repair.

Active FAP alpha is a ~170 kDa homodimeric protein which can also exist as a soluble form. FAP alpha can also form heterodimeric complexes with CD26 (DPP4), and these proteins may function co-ordinately to modulate the growth, differentiation, adhesion and metastasis of tumour cells.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to FAP alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide: extracellular domain of human FAP alpha, conjugated to keyhole limpet hemocyanin.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Uterus

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Further Reading

  1. Kelly, T. (2005) Fibroblast activation protein-alpha and dipeptidyl peptidase IV (CD26): cell-surface proteases that activate cell signaling and are potential targets for cancer therapy.
    Drug Resist Updat. 8 (1-2): 51-8.

Synonyms
SEPRASE
RRID
AB_2102362
UniProt
Q12884
Entrez Gene
FAP
GO Terms
GO:0004252 serine-type endopeptidase activity
GO:0008239 dipeptidyl-peptidase activity
GO:0004222 metalloendopeptidase activity
GO:0016021 integral to membrane
GO:0006508 proteolysis
GO:0010716 negative regulation of extracellular matrix disassembly
GO:0030054 cell junction
GO:0031258 lamellipodium membrane
GO:0042803 protein homodimerization activity
GO:0043542 endothelial cell migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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