Erythropoietin antibody

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Rabbit anti Human Erythropoietin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
4297-4955
Datasheet
E FN P WB
MSDS
0.1 mg loader

Rabbit anti Human erythropoietin antibody reacts with erythropoietin, the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological levels of circulating erythrocyte mass. It is often used as a drug to treat anemia.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Trehalose
Immunogen
CHO cell-derived, recombinant human erythropoietin.
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P01588 Related reagents
Entrez Gene
EPO Related reagents
GO Terms
GO:0007165 signal transduction
GO:0005128 erythropoietin receptor binding
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0008015 blood circulation
GO:0008284 positive regulation of cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043193 positive regulation of gene-specific transcription
GO:0043249 erythrocyte maturation
GO:0045740 positive regulation of DNA replication
GO:0046579 positive regulation of Ras protein signal transduction
Regulatory
For research purposes only

Applications of Erythropoietin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 µg/ml 1 µg/ml
Functional Assays
Immunohistology - Paraffin 15 µg/ml
Western Blotting 1 µg/ml 2 µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for Erythropoietin antibody

  1. Kessler, S.W. (1975) Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
    J Immunol. 115 (6): 1617-24.
  2. Krystal, G. (1983) A simple microassay for erythropoietin based on 3H-thymidine incorporation into spleen cells from phenylhydrazine treated mice.
    Exp Hematol. 11 (7): 649-60.