Erythropoietin antibody
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Rabbit anti Human erythropoietin antibody reacts with erythropoietin, the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological levels of circulating erythrocyte mass. It is often used as a drug to treat anemia.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <5% Trehalose
- Immunogen
- CHO cell-derived, recombinant human erythropoietin.
- Approx. Protein Concentrations
- 1.0 mg/ml
Storage Information
- Storage
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01588
- Entrez Gene
- EPO
- GO Terms
- GO:0007165 signal transduction
- GO:0005128 erythropoietin receptor binding
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0008015 blood circulation
- GO:0008284 positive regulation of cell proliferation
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0043193 positive regulation of gene-specific transcription
- GO:0043249 erythrocyte maturation
- GO:0045740 positive regulation of DNA replication
- GO:0046579 positive regulation of Ras protein signal transduction
- Regulatory
- For research purposes only
Applications of Erythropoietin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 µg/ml | 1 µg/ml | |
| Functional Assays | |||
| Immunohistology - Paraffin | 15 µg/ml | ||
| Western Blotting | 1 µg/ml | 2 µg/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Erythropoietin antibody
-
Kessler, S.W. (1975) Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
J Immunol. 115 (6): 1617-24. -
Krystal, G. (1983) A simple microassay for erythropoietin based on 3H-thymidine incorporation into spleen cells from phenylhydrazine treated mice.
Exp Hematol. 11 (7): 649-60.
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