ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody

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Rabbit anti ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2608 WB 50 µl
Rabbit anti ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody recognizes ERK1 when phosphorylated at threonine 202/tyrosine 204 and ERK2 when phosphorylated at threonine 185/tyrosine 187. ERK1 and ERK2 are members of the Mitogen Activated Protein Kinase (MAPK) family. MAPKs constitute a diverse set of signaling pathways. In the case of ERK a cascade is initiated by binding activation of a membrane bound receptor which causes activation of a small GTPase, RAS, which in turn activates the protein kinase activity of the RAF protein. RAF in turn phosphorylates and activates MEK. MEK induces phosphorylation of ERK1 at residues threonine 202/tyrosine 204 and ERK 2 at residues threonine 185/tyrosine 187. Phosphorylation of the residues corresponds to activation of ERK1/2 and mediation of its diverse biological functions including cell growth, differentiation, motility, and adhesion via its effects on transcription, translation, cytoskeletal rearrangement and cross talk with other signaling pathways.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ERK1/2 (pThr202/pTyr204) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 202/tyrosine 204 of human ERK1 and residues surrounding threonine 185/tyrosine 187 of human ERK2

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P27361 Related reagents
P28482 Related reagents
Entrez Gene
MAPK3 Related reagents
MAPK1 Related reagents
GO Terms
GO:0000186 activation of MAPKK activity
GO:0000187 activation of MAPK activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004707 MAP kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006361 transcription initiation from RNA polymerase I promoter
GO:0007049 cell cycle
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0030168 platelet activation
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade
GO:0003677 DNA binding
GO:0006350 transcription
GO:0006917 induction of apoptosis
GO:0007268 synaptic transmission
GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
GO:0070371 ERK1 and ERK2 cascade
Regulatory
For research purposes only

Applications of ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*