Rabbit anti ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody recognizes ERK1 when phosphorylated at threonine 202/tyrosine 204 and ERK2 when phosphorylated at threonine 185/tyrosine 187. ERK1 and ERK2 are members of the Mitogen Activated Protein Kinase (MAPK) family. MAPKs constitute a diverse set of signaling pathways. In the case of ERK a cascade is initiated by binding activation of a membrane bound receptor which causes activation of a small GTPase, RAS, which in turn activates the protein kinase activity of the RAF protein. RAF in turn phosphorylates and activates MEK. MEK induces phosphorylation of ERK1 at residues threonine 202/tyrosine 204 and ERK 2 at residues threonine 185/tyrosine 187. Phosphorylation of the residues corresponds to activation of ERK1/2 and mediation of its diverse biological functions including cell growth, differentiation, motility, and adhesion via its effects on transcription, translation, cytoskeletal rearrangement and cross talk with other signaling pathways.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ERK1/2 (pThr202/pTyr204) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 202/tyrosine 204 of human ERK1 and residues surrounding threonine 185/tyrosine 187 of human ERK2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPKK activity
activation of MAPK activity
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
MAP kinase activity
transcription initiation from RNA polymerase I promoter
epidermal growth factor receptor signaling pathway
Ras protein signal transduction
Toll signaling pathway
insulin receptor signaling pathway
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
interspecies interaction between organisms
innate immune response
nerve growth factor receptor signaling pathway
regulation of transcription factor activity
stress-activated MAPK cascade
induction of apoptosis
RNA polymerase II carboxy-terminal domain kinase activity
ERK1 and ERK2 cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available