Rabbit anti DFF-45 antibody recognizes DFF, also known as DNA fragmentation factor subunit alpha, DFF1, DFFA, inhibitor of caspase-activated DNase or ICAD.
DNA fragmentation factor (DFF) is a heterodimeric protein of 40kDa (DFF-40) and 45 kDa (DFF-45) subunits. During apoptosis caspase-3 cleaves DFF into the DFF-45 and DFF-40 fragments.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to DFF-45 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant human DFF-45
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
intracellular signal transduction
DNA fragmentation involved in apoptotic nuclear change
identical protein binding
negative regulation of apoptosis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of DFF-45 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti DFF-45 antibody detects a band of approximately 43 kDa in cell lysates and tissue lysates under reducing conditions
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Secondary Antibodies Available
Useful Reagents Available
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