DFF-45 antibody

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Rabbit anti DFF-45

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2459 P WB 50 µl
Rabbit anti DFF-45 antibody recognizes DFF, also known as DNA fragmentation factor subunit alpha, DFF1, DFFA, inhibitor of caspase-activated DNase or ICAD.

DNA fragmentation factor (DFF) is a heterodimeric protein of 40kDa (DFF-40) and 45 kDa (DFF-45) subunits. During apoptosis caspase-3 cleaves DFF into the DFF-45 and DFF-40 fragments.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to DFF-45 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human DFF-45

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
O00273 Related reagents
Entrez Gene
DFFA Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005739 mitochondrion
GO:0004536 deoxyribonuclease activity
GO:0005829 cytosol
GO:0035556 intracellular signal transduction
GO:0005654 nucleoplasm
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0042802 identical protein binding
GO:0043066 negative regulation of apoptosis
Regulatory
For research purposes only

Applications of DFF-45 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/200
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti DFF-45 antibody detects a band of approximately 43 kDa in cell lysates and tissue lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*