Rabbit antiuman DAD1 antibody recognizes defender of apoptotic cell death 1 (DAD1).
DAD1 was discovered as a negative regulator of programmed cell death, and has since been identified as a subunit of the mammalian oligosaccharyltransferase complex (OCT). DAD1 is required for the function and structural integrity of the OCT.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human DAD1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Synthetic peptide corresponding to a fourteen amino acid sequence within the C-Terminal region of human DAD1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
dolichyl-diphosphooligosaccharide-protein glycotransferase activity
protein N-linked glycosylation via asparagine
post-translational protein modification
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of DAD1 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP940 detects a band of approximately 22kDa in HepG2 cell lysates.
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Secondary Antibodies Available
Useful Reagents Available