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CTCF antibody

Rabbit anti Human CTCF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CTCF

Product Code Applications Pack Size List Price Your Price Qty
AHP3035
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
Ch IF P WB 0.1 ml loader
List Price Your Price
loader

Rabbit anti Human CTCF antibody recognizes CCCTC-binding factor, also known as 11-zinc finger protein.

CTCF is involved in many cellular processes, including regulation of transcription and 3D chromatin structure, thereby regulating expression of several genes, including insulin-like growth factor 2 (Phillips and Corces, 2009).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CTCF was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide within the N-terminal region of human CTCF
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation 2 µl per ChIP 8 µl per ChIP
Immunofluorescence 1/2000
Immunohistology - Paraffin 1/1000
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P49711
Entrez Gene
CTCF
GO Terms
GO:0007059 chromosome segregation
GO:0005515 protein binding
GO:0000793 condensed chromosome
GO:0000775 chromosome, centromeric region
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003714 transcription corepressor activity
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0043035 chromatin insulator sequence binding
GO:0008270 zinc ion binding
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016568 chromatin modification
GO:0016584 nucleosome positioning
GO:0040030 regulation of molecular function, epigenetic
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0070602 regulation of centromeric sister chromatid cohesion

AHP3035

160784

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
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