Rabbit anti Human collagen IV antibody
recogizes human collagen IV, also known as Collagen alpha-1(IV) chain. Collagen IV is a 1497 amino acid structural extracellular matrix protein and key component of the glomerular basement membrane. Arresten, comprising the c-terminal 225 amino acids of collagen IV demonstrates potent anti tumorigenic and anti angiogenic proterties (Nyberg et al. 2008
Rabbit anti Human collagen IV antibody exhibits the following reactivities with Collagen IV and related proteins:
|Human collagen IV||100%|
|Human types I, III and V||< 10%|
|Human collagen II||<1%|
Human plasma proteins do not interfere with collagen binding.
Defects in the COL4A1 gene encoding collagen 4 can lead to the presentation of a variety of conditions including Brain small vessel disease with or without ocular anomalies (BSVD
), demonstrating weakened brain blood vessels and retinal arteriolar tortuosity (Shah et al. 2010
). Hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC
), characterized by large renal cysts and hematuria (Plaisier et al. 2010
). Porencephaly 1 (POREN1
), a neurologic disease characterized by fluid filled cysts within the cerebral hemispheres, visual defect and facial paresis (Gould et al. 2005
). Schizencephaly (SCHZC
) presenting with full thickness cleft within the cereral hemispheres, some of the hemispheres may also be absent, replaced by cerebrospinal fluid (Yoneda et al. 2013
). Other conditions resulting from mutations in the COL4A1 gene also exist presenting with some of the features described for the above conditions.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Collagen IV antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin 1
- 1 Pretreatment is required with 1 mg/ml pepsin in 0.5 M acetic acid for two hours at 37ºC and multiple buffer washes.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.