Collagen IV antibody

Rabbit anti Human Collagen IV

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Collagen IV

Product Code Applications Pack Size List Price Your Price Qty
2150-0140
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF P * 0.5 ml
List Price Your Price
Search for Batch Specific Datasheets

Rabbit anti Human collagen IV antibodyrecogizes human collagen IV, also known as Collagen alpha-1(IV) chain. Collagen IV is a 1497 amino acid structural extracellular matrix protein and key component of the glomerular basement membrane. Arresten, comprising the c-terminal 225 amino acids of collagen IV demonstrates potent anti tumorigenic and anti angiogenic proterties (Nyberg et al. 2008).
Rabbit anti Human collagen IV antibody exhibits the following reactivities with Collagen IV and related proteins:

Human collagen IV100%
Human types I, III and V< 10%
Human collagen II<1%

Human plasma proteins do not interfere with collagen binding.

Defects in the COL4A1 gene encoding collagen 4 can lead to the presentation of a variety of conditions including Brain small vessel disease with or without ocular anomalies (BSVD), demonstrating weakened brain blood vessels and retinal arteriolar tortuosity (Shah et al. 2010). Hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC), characterized by large renal cysts and hematuria (Plaisier et al. 2010). Porencephaly 1 (POREN1), a neurologic disease characterized by fluid filled cysts within the cerebral hemispheres, visual defect and facial paresis (Gould et al. 2005). Schizencephaly (SCHZC) presenting with full thickness cleft within the cereral hemispheres, some of the hemispheres may also be absent, replaced by cerebrospinal fluid (Yoneda et al. 2013). Other conditions resulting from mutations in the COL4A1 gene also exist presenting with some of the features described for the above conditions.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Use 500 µl sterile distilled water.
Preparation
Purified IgG prepared by affinity chromatography on immobilized human collagen type IV
Buffer Solution
0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate
Preservative Stabilisers
1% Mannitol
1% Dextran
Immunogen
Collagen type IV from human placenta.
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at -20oC only.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1.5K 1/6K
Immunofluorescence 1/10 1/50
Immunohistology - Frozen 1/10 1/50
Immunohistology - Paraffin 1 1/10 1/50
  1. 1 Pretreatment is required with 1 mg/ml pepsin in 0.5 M acetic acid for two hours at 37ºC and multiple buffer washes.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Collagen IV antibody

  1. Jones. E.A.V. et al. (2008) Separating genetic and hemodynamic defects in neuropilin 1 knockout embryos.
    Development. 135: 2479-88.
  2. Cunningham, K. et al. (2011) Conditional deletion of Ccm2 causes hemorrhage in the adult brain: a mouse model of human cerebral cavernous malformations.
    Hum Mol Genet. 20: 3198-206.
  3. Reis, M. et al. (2012) Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression.
    J Exp Med. 209: 1611-27.
  4. Powner, M.B. et al. (2010) Perifoveal müller cell depletion in a case of macular telangiectasia type 2.
    Ophthalmology. 117: 2407-16.
  5. Dunér, P. et al. (2015) Increased aldehyde-modification of collagen type IV in symptomatic plaques - A possible cause of endothelial dysfunction.
    Atherosclerosis. 240 (1): 26-32.
  6. Powner, M.B. et al. (2016) Evaluation of Nonperfused Retinal Vessels in Ischemic Retinopathy.
    Invest Ophthalmol Vis Sci. 57 (11): 5031-7.

Immunofluorescence

RRID
AB_2082644
UniProt
P02462
P08572
Q01955
P53420
P29400
Q14031
Entrez Gene
COL4A1
COL4A2
COL4A5
COL4A4
COL4A3
COL4A6
GO Terms
GO:0001525 angiogenesis
GO:0005201 extracellular matrix structural constituent
GO:0007411 axon guidance
GO:0048407 platelet-derived growth factor binding
GO:0005515 protein binding
GO:0005587 collagen type IV
GO:0016525 negative regulation of angiogenesis
GO:0030198 extracellular matrix organization
GO:0005605 basal lamina
GO:0032836 glomerular basement membrane development
View more products with COLLAGEN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products