Collagen IV antibody

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Rabbit anti Human Collagen IV

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-0140 C E IF P* 0.5 ml
Rabbit anti Human collagen IV antibodyrecogizes human collagen IV, also known as Collagen alpha-1(IV) chain. Collagen IV is a 1497 amino acid structural extracellular matrix protein and key component of the glomerular basement membrane. Arresten, comprising the c-terminal 225 amino acids of collagen IV demonstrates potent anti tumorigenic and anti angiogenic proterties (Nyberg et al. 2008).
Rabbit anti Human collagen IV antibody exhibits the following reactivities with Collagen IV and related proteins:

Human collagen IV100%
Human types I, III and V< 10%
Human collagen II<1%

Human plasma proteins do not interfere with collagen binding.

Defects in the COL4A1 gene encoding collagen 4 can lead to the presentation of a variety of conditions including Brain small vessel disease with or without ocular anomalies (BSVD), demonstrating weakened brain blood vessels and retinal arteriolar tortuosity (Shah et al. 2010). Hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC), characterized by large renal cysts and hematuria (Plaisier et al. 2010). Porencephaly 1 (POREN1), a neurologic disease characterized by fluid filled cysts within the cerebral hemispheres, visual defect and facial paresis (Gould et al. 2005). Schizencephaly (SCHZC) presenting with full thickness cleft within the cereral hemispheres, some of the hemispheres may also be absent, replaced by cerebrospinal fluid (Yoneda et al. 2013). Other conditions resulting from mutations in the COL4A1 gene also exist presenting with some of the features described for the above conditions.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Use 500 µl sterile distilled water.
Preparation
Purified IgG prepared by affinity chromatography on immobilized human collagen type IV
Preservative Stabilisers
Contains mannitol, dextran and salts
Immunogen
Collagen type IV from human placenta.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P02462 Related reagents
P08572 Related reagents
Q01955 Related reagents
P53420 Related reagents
P29400 Related reagents
Q14031 Related reagents
Entrez Gene
COL4A1 Related reagents
COL4A2 Related reagents
COL4A5 Related reagents
COL4A4 Related reagents
COL4A3 Related reagents
COL4A6 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0005201 extracellular matrix structural constituent
GO:0007411 axon guidance
GO:0048407 platelet-derived growth factor binding
GO:0005515 protein binding
GO:0005587 collagen type IV
GO:0016525 negative regulation of angiogenesis
GO:0030198 extracellular matrix organization
GO:0005605 basal lamina
GO:0032836 glomerular basement membrane development
Regulatory
For research purposes only

Applications of Collagen IV antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1.5K 1/6K
Immunofluorescence 1/10 1/50
Immunohistology - Frozen 1/10 1/50
Immunohistology - Paraffin 1 1/10 1/50
  1. 1 Pretreatment is required with 1 mg/ml pepsin in 0.5 M acetic acid for two hours at 37ºC and multiple buffer washes.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Application Based External Images

Immunofluorescence

Product Specific References

References for Collagen IV antibody

  1. Jones. E.A.V. et al. (2008) Separating genetic and hemodynamic defects in neuropilin 1 knockout embryos.
    Development. 135: 2479-88.
  2. Cunningham, K. et al. (2011) Conditional deletion of Ccm2 causes hemorrhage in the adult brain: a mouse model of human cerebral cavernous malformations.
    Hum Mol Genet. 20: 3198-206.
  3. Reis, M. et al. (2012) Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression.
    J Exp Med. 209: 1611-27.
  4. Powner, M.B. et al. (2010) Perifoveal müller cell depletion in a case of macular telangiectasia type 2.
    Ophthalmology. 117: 2407-16.
  5. Dunér, P. et al. (2015) Increased aldehyde-modification of collagen type IV in symptomatic plaques - A possible cause of endothelial dysfunction.
    Atherosclerosis. 240 (1): 26-32.
  6. Powner, M.B. et al. (2016) Evaluation of Nonperfused Retinal Vessels in Ischemic Retinopathy.
    Invest Ophthalmol Vis Sci. 57 (11): 5031-7.