Collagen III antibody

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Rabbit anti Human Collagen III

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
2150-0100 Datasheet	C E P SDS	0.5 ml	Log in		Get Quote

Rabbit anti Human collagen III antibody recognizes human collagen type III. It has shown the following cross-reactivities.

Human collagen type I	<15%
Human collagen type IV	<5%
Human collagen type II and V	<1%

Human plasma proteins do not interfere with binding of the antibody to collagen.

Product Details

Target Species: Human
Product Form: Purified IgG - lyophilized
Reconstitution: Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation: Antiserum to native and denatured human collagen type III was raised by repeated immunisation of rabbits with highly purified antigen. 2150-0100 has been cross-absorbed with immobilised human collagen types I, IV and V and with human blood plasma proteins.
Buffer Solution: 0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate
Preservative Stabilisers: 1% Mannitol
1% Dextran
Immunogen: Collagen III from human placenta.
Approx. Protein Concentrations: 0.1 mg/ml after reconstitution

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee: Guaranteed until date of expiry. Please see product label.

More Information

UniProt: P02461
Entrez Gene: COL3A1
Regulatory: For research purposes only

Applications of Collagen III antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/2000	1/8000
Immunohistology - Frozen
Immunohistology - Paraffin	1/10	1/40

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in		Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in		Get Quote

Product Specific References

References for Collagen III antibody

George, C.L. et al. (2001) Endotoxin responsiveness and subchronic grain dust-induced airway disease.
Am J Physiol Lung Cell Mol Physiol. 280 (2): L203-13.
von Zur Muhlen, C. et al. (2009) Evaluation of urine proteome pattern analysis for its potential to reflect coronary artery atherosclerosis in symptomatic patients.
J Proteome Res. 8 (1): 335-45.
Zorin, V,L, et al. (2014) Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.
Biomed Mater. 9: 055005.
Zorin, V. et al. (2017) Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.
J Tissue Eng Regen Med. 11 (3): 778-86.
El-Domyati, M. et al. (2002) Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin.
Exp Dermatol. 11: 398-405.
El-Domyati, M.B. et al. (2004) Trichloroacetic acid peeling versus dermabrasion: a histometric, immunohistochemical, and ultrastructural comparison.
Dermatol Surg. 30: 179-88.
Iglezias, S.D. et al. (2008) Endomyocardial fibrosis: pathological and molecular findings of surgically resected ventricular endomyocardium.
Virchows Arch. 453: 233-41.
El-Domyati, M.M. et al. (2004) Effect of topical tretinoin on photoaged facial skin: a histometric, immunohistochemical and ultrastructural study.
J Cosmet Dermatol. 3: 191-201.
Sammons, R.L. et al. (2002) Histochemical, immunohistological and scanning electron microscope analysis of tissue retained on spontaneously extruded ventilation tubes.
J Laryngol Otol. 116: 333-9.

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