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Collagen III antibody

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Rabbit anti Human Collagen III

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
2150-0100
Datasheet
C E P
SDS
0.5 ml loader

Rabbit anti Human collagen III antibody recognizes human collagen type III. It has shown the following cross-reactivities.

Human collagen type I<15%
Human collagen type IV<5%
Human collagen type II and V<1%

Human plasma proteins do not interfere with binding of the antibody to collagen.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to native and denatured human collagen type III was raised by repeated immunisation of rabbits with highly purified antigen. 2150-0100 has been cross-absorbed with immobilised human collagen types I, IV and V and with human blood plasma proteins.
Buffer Solution
Phosphate/Borate buffered saline.
Preservative Stabilisers
1% Dextran
1% Mannitol
Immunogen
Collagen III from human placenta.
Approx. Protein Concentrations
0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
12 months from date of despatch

More Information

UniProt
P02461
Entrez Gene
COL3A1
Regulatory
For research purposes only

Applications of Collagen III antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/8000
Immunohistology - Frozen
Immunohistology - Paraffin 1/10 1/40
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Product Specific References

References for Collagen III antibody

  1. George, C.L. et al. (2001) Endotoxin responsiveness and subchronic grain dust-induced airway disease.
    Am J Physiol Lung Cell Mol Physiol. 280 (2): L203-13.
  2. von Zur Muhlen, C. et al. (2009) Evaluation of urine proteome pattern analysis for its potential to reflect coronary artery atherosclerosis in symptomatic patients.
    J Proteome Res. 8 (1): 335-45.
  3. Zorin, V,L, et al. (2014) Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.
    Biomed Mater. 9: 055005.
  4. Zorin, V. et al. (2017) Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.
    J Tissue Eng Regen Med. 11 (3): 778-86.
  5. El-Domyati, M. et al. (2002) Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin.
    Exp Dermatol. 11: 398-405.
  6. El-Domyati, M.B. et al. (2004) Trichloroacetic acid peeling versus dermabrasion: a histometric, immunohistochemical, and ultrastructural comparison.
    Dermatol Surg. 30: 179-88.
  7. Iglezias, S.D. et al. (2008) Endomyocardial fibrosis: pathological and molecular findings of surgically resected ventricular endomyocardium.
    Virchows Arch. 453: 233-41.
  8. El-Domyati, M.M. et al. (2004) Effect of topical tretinoin on photoaged facial skin: a histometric, immunohistochemical and ultrastructural study.
    J Cosmet Dermatol. 3: 191-201.
  9. Sammons, R.L. et al. (2002) Histochemical, immunohistological and scanning electron microscope analysis of tissue retained on spontaneously extruded ventilation tubes.
    J Laryngol Otol. 116: 333-9.
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