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Collagen III antibody

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Rabbit anti Human Collagen III

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Collagen III

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Product Code Applications Pack Size List Price Your Price Qty
2150-0100
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E P 0.5 ml
List Price Your Price

Rabbit anti Human collagen III antibody recognizes human collagen type III. It has shown the following cross-reactivities.

Human collagen type I<15%
Human collagen type IV<5%
Human collagen type II and V<1%

Human plasma proteins do not interfere with binding of the antibody to collagen.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to native and denatured human collagen type III was raised by repeated immunisation of rabbits with highly purified antigen. 2150-0100 has been cross-absorbed with immobilised human collagen types I, IV and V and with human blood plasma proteins.
Buffer Solution
0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate
Preservative Stabilisers
1% Mannitol
1% Dextran
Immunogen
Collagen III from human placenta.
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/8000
Immunohistology - Frozen
Immunohistology - Paraffin 1/10 1/40
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Collagen III antibody

  1. George, C.L. et al. (2001) Endotoxin responsiveness and subchronic grain dust-induced airway disease.
    Am J Physiol Lung Cell Mol Physiol. 280 (2): L203-13.
  2. von Zur Muhlen, C. et al. (2009) Evaluation of urine proteome pattern analysis for its potential to reflect coronary artery atherosclerosis in symptomatic patients.
    J Proteome Res. 8 (1): 335-45.
  3. Zorin, V,L, et al. (2014) Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.
    Biomed Mater. 9: 055005.
  4. Zorin, V. et al. (2017) Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.
    J Tissue Eng Regen Med. 11 (3): 778-86.
  5. El-Domyati, M. et al. (2002) Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin.
    Exp Dermatol. 11: 398-405.
  6. El-Domyati, M.B. et al. (2004) Trichloroacetic acid peeling versus dermabrasion: a histometric, immunohistochemical, and ultrastructural comparison.
    Dermatol Surg. 30: 179-88.
  7. Iglezias, S.D. et al. (2008) Endomyocardial fibrosis: pathological and molecular findings of surgically resected ventricular endomyocardium.
    Virchows Arch. 453: 233-41.
  8. El-Domyati, M.M. et al. (2004) Effect of topical tretinoin on photoaged facial skin: a histometric, immunohistochemical and ultrastructural study.
    J Cosmet Dermatol. 3: 191-201.
    9. View The Latest Product References
  9. Sammons, R.L. et al. (2002) Histochemical, immunohistological and scanning electron microscope analysis of tissue retained on spontaneously extruded ventilation tubes.
    J Laryngol Otol. 116: 333-9.

RRID
AB_620309
UniProt
P02461
Entrez Gene
COL3A1

2150-0100

157168 157405 161889 261114

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