Collagen III antibody
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Rabbit anti Human collagen III antibody recognizes human collagen type III. It has shown the following cross-reactivities.
Human plasma proteins do not interfere with binding of the antibody to collagen.
| Human collagen type I | <15% |
| Human collagen type IV | <5% |
| Human collagen type II and V | <1% |
Human plasma proteins do not interfere with binding of the antibody to collagen.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to native and denatured human collagen type III was raised by repeated immunisation of rabbits with highly purified antigen. 2150-0100 has been cross-absorbed with immobilised human collagen types I, IV and V and with human blood plasma proteins.
- Buffer Solution
- Phosphate/Borate buffered saline.
- Preservative Stabilisers
- 1% Dextran
1% Mannitol - Immunogen
- Collagen III from human placenta.
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P02461 Related reagents
- Entrez Gene
- COL3A1 Related reagents
- Regulatory
- For research purposes only
Applications of Collagen III antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | 1/8000 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | 1/10 | 1/40 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P |
Product Specific References
References for Collagen III antibody
-
George, C.L. et al. (2001) Endotoxin responsiveness and subchronic grain dust-induced airway disease.
Am J Physiol Lung Cell Mol Physiol. 280 (2): L203-13. -
von Zur Muhlen, C. et al. (2009) Evaluation of urine proteome pattern analysis for its potential to reflect coronary artery atherosclerosis in symptomatic patients.
J Proteome Res. 8 (1): 335-45. -
Zorin, V,L, et al. (2014) Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.
Biomed Mater. 9: 055005. -
Zorin, V. et al. (2017) Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.
J Tissue Eng Regen Med. 11 (3): 778-86. -
El-Domyati, M. et al. (2002) Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin.
Exp Dermatol. 11: 398-405. -
El-Domyati, M.B. et al. (2004) Trichloroacetic acid peeling versus dermabrasion: a histometric, immunohistochemical, and ultrastructural comparison.
Dermatol Surg. 30: 179-88. -
Iglezias, S.D. et al. (2008) Endomyocardial fibrosis: pathological and molecular findings of surgically resected ventricular endomyocardium.
Virchows Arch. 453: 233-41. -
El-Domyati, M.M. et al. (2004) Effect of topical tretinoin on photoaged facial skin: a histometric, immunohistochemical and ultrastructural study.
J Cosmet Dermatol. 3: 191-201. -
Sammons, R.L. et al. (2002) Histochemical, immunohistological and scanning electron microscope analysis of tissue retained on spontaneously extruded ventilation tubes.
J Laryngol Otol. 116: 333-9.
