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Collagen I/II/III/IV/V antibody

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Rabbit anti Human Collagen I/II/III/IV/V

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Collagen I/II/III/IV/V

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
2150-2206
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF 50 µg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human collagen I/II/III/IV/V antibodyrecognizes multiple collagen types and exhibits the following reactivities in ELISA with both native and denaturated human collagen types:

I & III100%
IV & V90%
II 80%

Human fibronectin, albumin and immunoglobulins all <0.5%

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified Ig prepared by affinity chromatography on immobilized collagen types I-V
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Mannitol
0.1%Dextran
0.09% Sodium Azide (NaN3)
Immunogen
A mixture of human collagen I, II, III, IV and V from human placenta.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/8000
Immunofluorescence 1/10 1/40
Immunohistology - Frozen 1/10 1/40
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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RRID
AB_620306
UniProt
P02452
P08123
P02458
P02461
P02462
P08572
Q01955
P53420
P29400
Q14031
P20908
P05997
P25940
Entrez Gene
COL1A1
COL2A1
COL3A1
COL4A1
COL5A2
COL1A2
COL4A2
COL5A1
COL5A3
COL4A5
COL4A4
COL4A3
COL4A6
GO Terms
GO:0001525 angiogenesis
GO:0005201 extracellular matrix structural constituent
GO:0007411 axon guidance
GO:0048407 platelet-derived growth factor binding
GO:0005515 protein binding
GO:0005587 collagen type IV
GO:0016525 negative regulation of angiogenesis
GO:0030198 extracellular matrix organization
GO:0007155 cell adhesion
GO:0005178 integrin binding
GO:0005588 collagen type V
GO:0008201 heparin binding
GO:0030199 collagen fibril organization
GO:0032964 collagen biosynthetic process
GO:0035313 wound healing, spreading of epidermal cells
GO:0043206 fibril organization
GO:0043394 proteoglycan binding
GO:0043588 skin development
GO:0045112 integrin biosynthetic process
GO:0048592 eye morphogenesis
GO:0005518 collagen binding
GO:0005605 basal lamina
GO:0032836 glomerular basement membrane development
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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