Collagen I/II/III/IV/V antibody
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Rabbit anti Human collagen I/II/III/IV/V antibodyrecognizes multiple collagen types and exhibits the following reactivities in ELISA with both native and denaturated human collagen types:
Human fibronectin, albumin and immunoglobulins all <0.5%
| I & III | 100% |
| IV & V | 90% |
| II | 80% |
Human fibronectin, albumin and immunoglobulins all <0.5%
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified Ig prepared by affinity chromatography on immobilized collagen types I-V
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3)0.1% Mannitol 0.1% Dextran - Immunogen
- A mixture of human collagen I, II, III, IV and V from human placenta.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P02452
- P08123
- P02458
- P02461
- P02462
- P08572
- Q01955
- P53420
- P29400
- Q14031
- P20908
- P05997
- P25940
- Entrez Gene
- COL1A1
- COL2A1
- COL3A1
- COL4A1
- COL5A2
- COL1A2
- COL4A2
- COL5A1
- COL5A3
- COL4A5
- COL4A4
- COL4A3
- COL4A6
- GO Terms
- GO:0001525 angiogenesis
- GO:0005201 extracellular matrix structural constituent
- GO:0007411 axon guidance
- GO:0048407 platelet-derived growth factor binding
- GO:0005515 protein binding
- GO:0005587 collagen type IV
- GO:0016525 negative regulation of angiogenesis
- GO:0030198 extracellular matrix organization
- GO:0007155 cell adhesion
- GO:0005178 integrin binding
- GO:0005588 collagen type V
- GO:0008201 heparin binding
- GO:0030199 collagen fibril organization
- GO:0032964 collagen biosynthetic process
- GO:0035313 wound healing, spreading of epidermal cells
- GO:0043206 fibril organization
- GO:0043394 proteoglycan binding
- GO:0043588 skin development
- GO:0045112 integrin biosynthetic process
- GO:0048592 eye morphogenesis
- GO:0005518 collagen binding
- GO:0005605 basal lamina
- GO:0032836 glomerular basement membrane development
- Regulatory
- For research purposes only
Applications of Collagen I/II/III/IV/V antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | 1/8000 | |
| Immunofluorescence | 1/10 | 1/40 | |
| Immunohistology - Frozen | 1/10 | 1/40 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
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