CENP-A (pSer18) antibody

Rabbit anti Human CENP-A (pSer18)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CENP-A (pSer18)

Product Code Applications Pack Size List Price Your Price Qty
AHP3033
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SDS Safety Datasheet SDS
IF WB 0.1 ml loader
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Rabbit anti human CENP-A (pSer18) antibody recognizes centromere protein A when phosphorylated at serine 18.

CENP-A is a histone H3 variant which replaces one or both canonical H3 histones in a subset of nucleosomes within centromeric chromatin and epigenetically determines the position of the centromere on each chromosome, and where kinetochore assembly and sister chromatid cohesion occurs during mitosis (Fachinetti et al. 2013) (Blower et al. 2002). CENP-A has been shown to interact directly with the inner kinetochore through CENP-C and CENP-N. This interaction allows microtubules to accurately segregate chromosomes during mitosis (Kixomoeller et al. 2020) (Yan et al. 2019). Phosphorylation of CENP-A on serine 18 is thought to play an important role in the segregation of chromosomes during mitosis (Kixomoeller et al. 2020) (Zhang et al. 2017).

Rabbit anti human CENP-A (pSer18) antibody shows minimal cross-reactivity with CENP-A that has been phosphorylated at serine 16.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CENP-A (pSer18) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/100
Western Blotting 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P49450
Entrez Gene
CENPA
GO Terms
GO:0000132 establishment of mitotic spindle orientation
GO:0005515 protein binding
GO:0000236 mitotic prometaphase
GO:0000778 condensed nuclear chromosome kinetochore
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0044419 interspecies interaction between organisms
GO:0051382 kinetochore assembly
GO:0071459 protein localization to chromosome, centromeric region
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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