CENP-A (pSer18) antibody
Rabbit anti Human CENP-A (pSer18)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CENP-A (pSer18)
Antibody Quality and Performance Guarantee
| Rabbit anti human CENP-A (pSer18) antibody recognizes centromere protein A when phosphorylated at serine 18. CENP-A is a histone H3 variant which replaces one or both canonical H3 histones in a subset of nucleosomes within centromeric chromatin and epigenetically determines the position of the centromere on each chromosome, and where kinetochore assembly and sister chromatid cohesion occurs during mitosis (Fachinetti et al. 2013) (Blower et al. 2002). CENP-A has been shown to interact directly with the inner kinetochore through CENP-C and CENP-N. This interaction allows microtubules to accurately segregate chromosomes during mitosis (Kixomoeller et al. 2020) (Yan et al. 2019). Phosphorylation of CENP-A on serine 18 is thought to play an important role in the segregation of chromosomes during mitosis (Kixomoeller et al. 2020) (Zhang et al. 2017). Rabbit anti human CENP-A (pSer18) antibody shows minimal cross-reactivity with CENP-A that has been phosphorylated at serine 16. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human CENP-A (pSer18) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- A synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
1/100 | |
| Western Blotting | |
1/100 | 1/500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
- UniProt
- P49450
- Entrez Gene
- CENPA
- GO Terms
- GO:0000132 establishment of mitotic spindle orientation
- GO:0005515 protein binding
- GO:0000236 mitotic prometaphase
- GO:0000778 condensed nuclear chromosome kinetochore
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0003682 chromatin binding
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- View More GO Terms
- GO:0034080 CenH3-containing nucleosome assembly at centromere
- GO:0044419 interspecies interaction between organisms
- GO:0051382 kinetochore assembly
- GO:0071459 protein localization to chromosome, centromeric region
AHP3033
160769
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