CDK8 antibody

Rabbit anti Human CDK8

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CDK8

Product Code Applications Pack Size List Price Your Price Qty
AHP3032
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
Ch WB 0.1 ml loader
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Rabbit anti Human CDK8 antibody recognizes cell division protein kinase 8, also known as cell division protein kinase 8 or mediator complex subunit CDK8.

CDK8 is a 53 kDa is a member of the cyclin-dependent protein kinase (CDK) family involved in transcriptional regulation via interaction with the mediator complex (Tassan et al. 1995). CDK8 regulates the activity of several transcription factors via its kinase activity, including STAT1 (Bancerek et al. 2013). CDK8 is also able to inhibit transcriptional activation by influencing the turnover of subunits in the mediator complex tail module and mediating the binding of RNA polymerase II to the mediator complex (Tsai et al. 2013). Expression of CDK8 may contribute to the development of a number of different types of cancer (Firestein et al. 2008, Donner et al. 2007).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CDK8 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide within the C-terminal region of human CDK8
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation 10 µl per ChIP
Western Blotting 1/500 1/1500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P49336
Entrez Gene
CDK8
GO Terms
GO:0005515 protein binding
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0006350 transcription
GO:0006468 protein phosphorylation
GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
GO:0016592 mediator complex
GO:0045449 regulation of transcription
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products