CD5L antibody

Rabbit anti CD5L (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD5L
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1168
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human CD5l antibody detects the C-terminal (CT) region of human CD5 like (CD5L), also known as Apoptosis Inhibitor expressed by Macrophages (AIM), and SP alpha. CD5L is a soluble ~38 kDa glycoprotein produced by mature macrophages, and has been shown to support their survival and enhance phagocytic function. CD5L expression is upregulated in response to LXR/RXR heterodimer activation.

CD5L has been shown to mediate the survival of macrophages in atherosclerotic plaques.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CD5L were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
13 amino acid peptide sequence near the carboxy terminus of human CD5L.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1168 detects a band of approximately 38kDa in Raji cell lysates.

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References for CD5L antibody

  1. Li, Y. et al. (2008) Extracellular Nampt promotes macrophage survival via a nonenzymatic interleukin-6/STAT3 signaling mechanism.
    J Biol Chem. 283 (50): 34833-43.

Further Reading

  1. Sarrias, M.R. et al. (2005) A role for human Sp alpha as a pattern recognition receptor.
    J Biol Chem. 280 (42): 35391-8.
  2. Arai, S. et al. (2005) A role for the apoptosis inhibitory factor AIM/Spalpha/Api6 in atherosclerosis development.
    Cell Metab. 1 (3): 201-13.

Western Blotting

Synonyms
SP-ALPHA
RRID
AB_808449
UniProt
O43866
Entrez Gene
CD5L
GO Terms
GO:0016020 membrane
GO:0006915 apoptosis
GO:0005044 scavenger receptor activity
GO:0005615 extracellular space
GO:0006968 cellular defense response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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