CD5L antibody

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Anti CD5L (C-Terminal) Antibody thumbnail image 1

Anti CD5L (C-Terminal) Antibody thumbnail image 2

Anti CD5L (C-Terminal) Antibody gallery image 1 — Western blot analysis of whole cell lysates from Raji human Burkitt's lymphoma cells probed with Rabbit anti Human CD5L, also known as AIM (**AHP1168**) at 0.5(A), 1(B) and 2(C) ug/ml.

Anti CD5L (C-Terminal) Antibody gallery image 2 — Formalin fixed, paraffin embedded human lymph node stained with Rabbit anti Human CD5L (**AHP1168**) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0).

Rabbit anti CD5L (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1168	P* WB			0.1 mg

Rabbit anti Human CD5l antibody detects the C-terminal (CT) region of human CD5 like (CD5L), also known as Apoptosis Inhibitor expressed by Macrophages (AIM), and SP alpha. CD5L is a soluble ~38 kDa glycoprotein produced by mature macrophages, and has been shown to support their survival and enhance phagocytic function. CD5L expression is upregulated in response to LXR/RXR heterodimer activation.

CD5L has been shown to mediate the survival of macrophages in atherosclerotic plaques.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human CD5L were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02%

Sodium Azide

Immunogen

13 amino acid peptide sequence near the carboxy terminus of human CD5L.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: O43866 Related reagents
Entrez Gene: CD5L Related reagents
GO Terms: GO:0016020 membrane; GO:0006915 apoptosis; GO:0005044 scavenger receptor activity; GO:0005615 extracellular space; GO:0006968 cellular defense response
Regulatory: For research purposes only

Applications of CD5L antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin ¹
Western Blotting		0.5	1.0ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1168 detects a band of approximately 38kDa in Raji cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

Application Based External Images

Western Blotting

Product Specific References

References for CD5L antibody

Li, Y. et al. (2008) Extracellular Nampt promotes macrophage survival via a nonenzymatic interleukin-6/STAT3 signaling mechanism.
J Biol Chem. 283 (50): 34833-43.