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CD5L antibody

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Anti CD5L (C-Terminal) Antibody thumbnail image 1 Anti CD5L (C-Terminal) Antibody thumbnail image 2
Anti CD5L (C-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysates from Raji human Burkitt's lymphoma cells probed with Rabbit anti Human CD5L, also known as AIM (AHP1168) at 0.5(A), 1(B) and 2(C) ug/ml.
Anti CD5L (C-Terminal) Antibody gallery image 2
Formalin fixed, paraffin embedded human lymph node stained with Rabbit anti Human CD5L (AHP1168) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0).

Rabbit anti CD5L (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1168
Datasheet
P* WB
SDS
0.1 mg loader

Rabbit anti Human CD5l antibody detects the C-terminal (CT) region of human CD5 like (CD5L), also known as Apoptosis Inhibitor expressed by Macrophages (AIM), and SP alpha. CD5L is a soluble ~38 kDa glycoprotein produced by mature macrophages, and has been shown to support their survival and enhance phagocytic function. CD5L expression is upregulated in response to LXR/RXR heterodimer activation.

CD5L has been shown to mediate the survival of macrophages in atherosclerotic plaques.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CD5L were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
13 amino acid peptide sequence near the carboxy terminus of human CD5L.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O43866
Entrez Gene
CD5L
GO Terms
GO:0016020 membrane
GO:0006915 apoptosis
GO:0005044 scavenger receptor activity
GO:0005615 extracellular space
GO:0006968 cellular defense response
Regulatory
For research purposes only

Applications of CD5L antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1168 detects a band of approximately 38kDa in Raji cell lysates.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
100x Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Western Blotting

Product Specific References

References for CD5L antibody

  1. Li, Y. et al. (2008) Extracellular Nampt promotes macrophage survival via a nonenzymatic interleukin-6/STAT3 signaling mechanism.
    J Biol Chem. 283 (50): 34833-43.

Further Reading

  1. Sarrias, M.R. et al. (2005) A role for human Sp alpha as a pattern recognition receptor.
    J Biol Chem. 280 (42): 35391-8.
  2. Arai, S. et al. (2005) A role for the apoptosis inhibitory factor AIM/Spalpha/Api6 in atherosclerosis development.
    Cell Metab. 1 (3): 201-13.

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