CD257 antibody
Rabbit anti Human CD257 antibody recognizes CD257, also known as BLyS, BAFF, TALL-1 and THANK. CD257 is a member of the tumor necrosis factor (TNF) superfamily. CD257 and its receptor TACI/BCMA are involved in the development of autoimmune diseases. CD257 activates NFκB and JNK (c-jun N-terminal kinase) inducing apoptosis.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to CD257 (BAFF) were raised by repeated immunisation of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Peptide corresponding to amino acids 254-269 of human CD257 (BAFF).
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Y275
- Entrez Gene
- TNFSF13B
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0007165 signal transduction
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006955 immune response
- GO:0008283 cell proliferation
- Regulatory
- For research purposes only
Applications of CD257 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunocytochemistry | 1ug/ml | ||
| Western Blotting | 0.25ug/ml | 1ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP587 detects a band of approximately 31kDa in mouse spleen tissue lysate and human HL60 cell lysate
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Moore, P.A. et al. (1999) BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator.
Science. 285 (5425): 260-3.
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