CD209 antibody

Rabbit anti Human CD209

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD209

Product Code Applications Pack Size List Price Your Price Qty
AHP627
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IF WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human CD209 antibody recognizes an epitope within the extracellular domain of human DC-SIGN. DC-SIGN was designated CD209 at the 7th leucocyte typing workshop.

Rabbit anti Human CD209 antibody detects a band of approximately 44 kDa by western blot.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human DC-SIGN were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 277-293 of human DC-SIGN.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Western Blotting 1ug/ml 2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for CD209 antibody

  1. Necchi, V. et al. (2009) Evidence for transepithelial dendritic cells in human H. pylori active gastritis.
    Helicobacter.14: 208-22.
  2. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
    Dev Comp Immunol. 32: 837-49.
  3. Olivier, M. et al. (2012) Capacities of Migrating CD1b Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
    PLoS One. 7: e30430.
  4. Emara, M. et al. (2012) Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.
    J Biol Chem. 287 (8): 5756-63.
  5. Piercey Åkesson C et al. (2012) Phenotypic characterization of cells participating in transport of prion protein aggregates across the intestinal mucosa of sheep.
    Prion. 6 (3): 261-75.

Further Reading

  1. Geijtenbeek, T. B. et al. (2000) DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells.
    Cell. 100: 587-97.

Immunofluorescence

Western Blotting

Synonyms
DC-SIGN
RRID
AB_323683
UniProt
Q9NNX6
Entrez Gene
CD209
View more products with CD209 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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