CCL25 antibody
Rabbit anti Human CCL25
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CCL25
Antibody Quality and Performance Guarantee
| Rabbit anti Human CCL25 antibody recognizes human CCL25, otherwise known as TECK (thymus expressed chemokine), a member of the intercrine beta (chemokine CC) family, expressed by thymic stromal cells, which acts as a chemotactic factor for activated macrophages, thymocytes and dendritic cells, and signals through binding to the CCR9 receptor. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antiserum to human CCL25 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human CCL25.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
10.0 | 15.0ug/ml |
| Immunohistology - Paraffin 1 | |
0.5 | 1.0ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
- Synonyms
- TECK
- RRID
- AB_2275290
- UniProt
- O15444
- Entrez Gene
- CCL25
- GO Terms
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0008009 chemokine activity
AHP1920
154802
159280
163727
165009
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