Caspase-9 antibody

Rabbit anti Human Caspase-9 (aa299-318)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Caspase-9
Region
(aa299-318)

Product Code Applications Pack Size List Price Your Price Qty
AHP492
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
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Rabbit anti Human caspase-9 antibody recognizes human caspase-9, also known as ICE-LAP6, McL6 and Apaf-3. Caspase-9 plays a central role in programmed cell death. Following activation, induced by the binding of Apaf-1, caspase-9 cleaves and activates caspase-3.

Rabbit anti Human caspase-9 antibody (AHP492) recognizes the ~46 kDa caspase-9 in western blots.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human caspase-9 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 299-318 of human caspase-9.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Li, P. et al. (1997) Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade.
    Cell. 91 (4): 479-89.
  2. Pan, G. et al. (1998) Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex.
    J Biol Chem. 273 (10): 5841-5.

RRID
AB_322898
UniProt
P55211
Entrez Gene
CASP9
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005829 cytosol
GO:0006508 proteolysis
GO:0008047 enzyme activator activity
GO:0008629 induction of apoptosis by intracellular signals
GO:0008635 activation of caspase activity by cytochrome c
GO:0048015 phosphatidylinositol-mediated signaling
GO:0048011 nerve growth factor receptor signaling pathway
View more products with CASPASE specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products