Caspase-8 antibody

Rabbit anti Caspase-8 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Caspase-8
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP967
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
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Search for Batch Specific Datasheets

Rabbit anti Human caspase-8 antibody recognizes an epitope within the c-terminal region (CT) of caspase-8, otherwise known as Fas-linked ICE-like protease (FLICE), which shares homology with both Fas-associated via death domain (FADD) and the ICE/Ced-3 family of cysteine proteases.

Caspase-8 is classified as an initiator caspase and acts as the most upstream trigger of Fas-Receptor (CD95) and tumour necrosis factor receptor 1 (TNFR1) mediated apoptosis. Upon activation, the cell death receptor Fas, links with the adaptor molecule FADD via corresponding death domains (DD). FADD in turn then binds via death effector domains (DEDs) to procaspase-8, which then undergoes oligomerization and autocatalytic activation.

Active Caspase-8 is capable of cleaving all other known caspases, and also cleaves the Bcl-2 family member BH3-interacting domain death agonist (BID), along with the Bcl-2 associated protein p28Bap31 (Wolf & Green 1999).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human caspase-8 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
A 16 amino acid peptide located near the carboxy-terminus of human caspase-8 isoform A.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP967 detects a cleaved subunit band of approximately 22.5kDa in Jurkat cell lysates (predicted precursor MWt 57.7kDa).

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References for Caspase-8 antibody

  1. Umezaki, Y. et al. (2011) Blocking synaptic transmission with tetanus toxin light chain reveals modes of neurotransmission in the PDF-positive circadian clock neurons of Drosophila melanogaster.
    J Insect Physiol. 57 (9): 1290-9.

Further Reading

  1. Muzio, M. et al. (1996) FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex.
    Cell. 85 (6): 817-27.
  2. Wolf, B.B. & Green, D.R. (1999) Suicidal tendencies: apoptotic cell death by caspase family proteinases.
    J Biol Chem. 274 (29): 20049-52.
  3. Cohen, G.M. (1997) Caspases: the executioners of apoptosis.
    Biochem J. 326 ( Pt 1): 1-16.

Synonyms
FLICE
RRID
AB_2069877
UniProt
Q14790
Entrez Gene
CASP8
View more products with CASPASE specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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