Caspase-1 antibody

Rabbit anti Human Caspase-1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Caspase-1
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP963
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SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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Rabbit anti Human Caspase-1 antibody recognizes an epitope within the C-terminal region (CT) of human Caspase-1, otherwise known as IL-1Beta converting enzyme. Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3).

Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms.

Regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases (Ghayur et al. 1997).

Rabbit anti Human Caspase-1 antibody detects a cleaved subunit band of approximately 21 kDa in human heart cell lysates (predicted precursor MWT 45.2kDa).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Antisera to human Caspase-1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
A 16 amino acid peptide located near human Caspase-1 carboxy terminus.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2.0ug/ml
Western Blotting 0.5ug - 1.0ug/ml
  1. 1This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human heart.

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References for Caspase-1 antibody

  1. Faner, R. et al. (2016) The inflammasome pathway in stable COPD and acute exacerbations.
    ERJ Open Research. 2 (3): 00002-2016-2-2016.

Further Reading

  1. Ghayur, T.et al. (1997) Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production.
    Nature. 386 (6625): 619-23.
  2. Fantuzzi, G. et al. (1998) Interleukin-18 regulation of interferon gamma production and cell proliferation as shown in interleukin-1beta-converting enzyme (caspase-1)-deficient mice.
    Blood. 91 (6): 2118-25.
  3. Kostura, M.J. et al. (1989) Identification of a monocyte specific pre-interleukin 1 beta convertase activity.
    Proc Natl Acad Sci U S A. 86 (14): 5227-31.
  4. Miura, M. et al. (1993) Induction of apoptosis in fibroblasts by IL-1 beta-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3.
    Cell. 75 (4): 653-60.

Synonyms
IL-1 Beta Converting Enzyme
RRID
AB_2069046
UniProt
P29466
Entrez Gene
CASP1
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0006508 proteolysis
GO:0008656 caspase activator activity
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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