Caspase-1 antibody

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Rabbit anti Human Caspase-1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP963 P* WB 0.1 mg
Rabbit anti Human Caspase-1 antibody recognizes an epitope within the C-terminal region (CT) of human Caspase-1, otherwise known as IL-1Beta converting enzyme. Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3).

Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms.

Regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases (Ghayur et al. 1997).

Rabbit anti Human Caspase-1 antibody detects a cleaved subunit band of approximately 21 kDa in human heart cell lysates (predicted precursor MWT 45.2kDa).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Antisera to human Caspase-1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
A 16 amino acid peptide located near human Caspase-1 carboxy terminus.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P29466 Related reagents
Entrez Gene
CASP1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0006508 proteolysis
GO:0008656 caspase activator activity
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
Regulatory
For research purposes only

Applications of Caspase-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2.0ug/ml
Western Blotting 0.5ug - 1.0ug/ml
  1. 1This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human heart.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for Caspase-1 antibody

  1. Faner, R. et al. (2016) The inflammasome pathway in stable COPD and acute exacerbations.
    <a rel="nofollow" href="http://openres.ersjournals.com/content/2/3/00002-2016"target="_blank">ERJ Open Research. 2 (3): 00002-2016-2-2016</a>.

Further Reading

  1. Ghayur, T.et al. (1997) Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production.
    Nature. 386 (6625): 619-23.
  2. Fantuzzi, G. et al. (1998) Interleukin-18 regulation of interferon gamma production and cell proliferation as shown in interleukin-1beta-converting enzyme (caspase-1)-deficient mice.
    Blood. 91 (6): 2118-25.
  3. Kostura, M.J. et al. (1989) Identification of a monocyte specific pre-interleukin 1 beta convertase activity.
    Proc Natl Acad Sci U S A. 86 (14): 5227-31.
  4. Miura, M. et al. (1993) Induction of apoptosis in fibroblasts by IL-1 beta-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3.
    Cell. 75 (4): 653-60.