Caspase-1 antibody
Rabbit anti Human Caspase-1 (C-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Caspase-1
- Region
- (C-TERMINAL)
Antibody Quality and Performance Guarantee
| Rabbit anti Human Caspase-1 antibody recognizes an epitope within the C-terminal region (CT) of human Caspase-1, otherwise known as IL-1Beta converting enzyme. Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3). Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms. Regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases (Ghayur et al. 1997). Rabbit anti Human Caspase-1 antibody detects a cleaved subunit band of approximately 21 kDa in human heart cell lysates (predicted precursor MWT 45.2kDa). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Antisera to human Caspase-1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- A 16 amino acid peptide located near human Caspase-1 carboxy terminus.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
2.0ug/ml | |
| Western Blotting | |
0.5ug - 1.0ug/ml |
- 1This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human heart.
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References for Caspase-1 antibody
-
Faner, R. et al. (2016) The inflammasome pathway in stable COPD and acute exacerbations.
ERJ Open Res. 2 (3): 00002-2016.
Further Reading
-
Kostura, M.J. et al. (1989) Identification of a monocyte specific pre-interleukin 1 beta convertase activity.
Proc Natl Acad Sci U S A. 86 (14): 5227-31. -
Miura, M. et al. (1993) Induction of apoptosis in fibroblasts by IL-1 beta-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3.
Cell. 75 (4): 653-60. -
Ghayur, T.et al. (1997) Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production.
Nature. 386 (6625): 619-23. -
Fantuzzi, G. et al. (1998) Interleukin-18 regulation of interferon gamma production and cell proliferation as shown in interleukin-1beta-converting enzyme (caspase-1)-deficient mice.
Blood. 91 (6): 2118-25.
- Synonyms
- IL-1 Beta Converting Enzyme
- RRID
- AB_2069046
- UniProt
- P29466
- Entrez Gene
- CASP1
- GO Terms
- GO:0005515 protein binding
- GO:0004197 cysteine-type endopeptidase activity
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0007165 signal transduction
- GO:0006508 proteolysis
- GO:0008656 caspase activator activity
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
AHP963
031214
155496
161012
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