BRCA1 (pSer988) antibody
Rabbit anti BRCA1 (pSer988) antibody recognizes BRCA1, also known as breast cancer type 1 susceptibility protein and RING finger protein 53, when phosphorylated at serine 988. Breast cancer susceptibility genes BRCA1 and BRCA2 are associated with hereditary breast and ovarian cancers. BRCA1 is a tumor suppressor gene encoding an E3 ubiquitin protein ligase that plays a major role in DNA damage repair (Roy et al. 2011).
BRCA1 is also involved in the regulation of multiple processes including transcription and cell cycle progression. BRCA1 co-localizes with Chk2 in nuclear foci, in response to DNA damage BCRA1 is phosphorylated at serine 988 by Chk2. This phosphorylation event results in the release and dispersion of BRCA1 and is suggested to be important for the function of BRCA1 in the DNA damage response (Lee et al. 2000).
BRCA1 is also involved in the regulation of multiple processes including transcription and cell cycle progression. BRCA1 co-localizes with Chk2 in nuclear foci, in response to DNA damage BCRA1 is phosphorylated at serine 988 by Chk2. This phosphorylation event results in the release and dispersion of BRCA1 and is suggested to be important for the function of BRCA1 in the DNA damage response (Lee et al. 2000).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to BRCA1 (pSer988) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 988 of human BRCA1
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P38398
- Entrez Gene
- BRCA1
- GO Terms
- GO:0007059 chromosome segregation
- GO:0005515 protein binding
- GO:0000724 double-strand break repair via homologous recombination
- GO:0003677 DNA binding
- GO:0003713 transcription coactivator activity
- GO:0003723 RNA binding
- GO:0030529 ribonucleoprotein complex
- GO:0006915 apoptosis
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005654 nucleoplasm
- GO:0006301 postreplication repair
- GO:0006359 regulation of transcription from RNA polymerase III promoter
- GO:0006633 fatty acid biosynthetic process
- GO:0006978 DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
- GO:0007049 cell cycle
- GO:0008270 zinc ion binding
- GO:0008274 gamma-tubulin ring complex
- GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis
- GO:0042802 identical protein binding
- GO:0010212 response to ionizing radiation
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0015631 tubulin binding
- GO:0016481 negative regulation of transcription
- GO:0016563 transcription activator activity
- GO:0030521 androgen receptor signaling pathway
- GO:0031398 positive regulation of protein ubiquitination
- GO:0031436 BRCA1-BARD1 complex
- GO:0031572 G2/M transition DNA damage checkpoint
- GO:0031625 ubiquitin protein ligase binding
- GO:0042127 regulation of cell proliferation
- GO:0043627 response to estrogen stimulus
- GO:0045717 negative regulation of fatty acid biosynthetic process
- GO:0045739 positive regulation of DNA repair
- GO:0046600 negative regulation of centriole replication
- GO:0050681 androgen receptor binding
- GO:0051865 protein autoubiquitination
- GO:0070531 BRCA1-A complex
- GO:0071681 cellular response to indole-3-methanol
- GO:0085020 protein K6-linked ubiquitination
- Regulatory
- For research purposes only
Applications of BRCA1 (pSer988) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Roy, R. et al. (2011) BRCA1 and BRCA2: different roles in a common pathway of genome protection.
Nat Rev Cancer. 12 (1): 68-78. -
Lee, J.S. et al. (2000) hCds1-mediated phosphorylation of BRCA1 regulates the DNA damage response.
Nature. 404 (6774): 201-4.
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