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BRCA1 (pSer988) antibody

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Anti BRCA1 (pSer988) Antibody thumbnail image 1
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Western blot analysis of extracts from HeLa cells untreated or treated with UV light using Rabbit anti BRCA1 (pSer988) antibody. Cells were treated with UV light for 15-30 minutes

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Rabbit anti BRCA1 (pSer988)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
BRCA1 (pSer988)

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AHP2561
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Rabbit anti BRCA1 (pSer988) antibody recognizes BRCA1, also known as breast cancer type 1 susceptibility protein and RING finger protein 53, when phosphorylated at serine 988. Breast cancer susceptibility genes BRCA1 and BRCA2 are associated with hereditary breast and ovarian cancers. BRCA1 is a tumor suppressor gene encoding an E3 ubiquitin protein ligase that plays a major role in DNA damage repair (Roy et al. 2011).

BRCA1 is also involved in the regulation of multiple processes including transcription and cell cycle progression. BRCA1 co-localizes with Chk2 in nuclear foci, in response to DNA damage BCRA1 is phosphorylated at serine 988 by Chk2. This phosphorylation event results in the release and dispersion of BRCA1 and is suggested to be important for the function of BRCA1 in the DNA damage response (Lee et al. 2000).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to BRCA1 (pSer988) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 988 of human BRCA1
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Roy, R. et al. (2011) BRCA1 and BRCA2: different roles in a common pathway of genome protection.
    Nat Rev Cancer. 12 (1): 68-78.
  2. Lee, J.S. et al. (2000) hCds1-mediated phosphorylation of BRCA1 regulates the DNA damage response.
    Nature. 404 (6774): 201-4.

UniProt
P38398
Entrez Gene
BRCA1
GO Terms
GO:0007059 chromosome segregation
GO:0005515 protein binding
GO:0000724 double-strand break repair via homologous recombination
GO:0003677 DNA binding
GO:0003713 transcription coactivator activity
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0006915 apoptosis
GO:0004842 ubiquitin-protein ligase activity
GO:0005654 nucleoplasm
GO:0006301 postreplication repair
GO:0006359 regulation of transcription from RNA polymerase III promoter
GO:0006633 fatty acid biosynthetic process
GO:0006978 DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
GO:0007049 cell cycle
GO:0008270 zinc ion binding
GO:0008274 gamma-tubulin ring complex
GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis
GO:0042802 identical protein binding
GO:0010212 response to ionizing radiation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0015631 tubulin binding
GO:0016481 negative regulation of transcription
GO:0016563 transcription activator activity
GO:0030521 androgen receptor signaling pathway
GO:0031398 positive regulation of protein ubiquitination
GO:0031436 BRCA1-BARD1 complex
GO:0031572 G2/M transition DNA damage checkpoint
GO:0031625 ubiquitin protein ligase binding
GO:0042127 regulation of cell proliferation
GO:0043627 response to estrogen stimulus
GO:0045717 negative regulation of fatty acid biosynthetic process
GO:0045739 positive regulation of DNA repair
GO:0046600 negative regulation of centriole replication
GO:0050681 androgen receptor binding
GO:0051865 protein autoubiquitination
GO:0070531 BRCA1-A complex
GO:0071681 cellular response to indole-3-methanol
GO:0085020 protein K6-linked ubiquitination
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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