Rabbit anti BRCA1 (pSer988) antibody
recognizes BRCA1, also known as breast cancer type 1 susceptibility protein and RING finger protein 53, when phosphorylated at serine 988. Breast cancer susceptibility genes BRCA1 and BRCA2 are associated with hereditary breast and ovarian cancers. BRCA1 is a tumor suppressor gene encoding an E3 ubiquitin protein ligase that plays a major role in DNA damage repair (Roy et al. 2011
BRCA1 is also involved in the regulation of multiple processes including transcription and cell cycle progression. BRCA1 co-localizes with Chk2 in nuclear foci, in response to DNA damage BCRA1 is phosphorylated at serine 988 by Chk2. This phosphorylation event results in the release and dispersion of BRCA1 and is suggested to be important for the function of BRCA1 in the DNA damage response (Lee et al. 2000
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to BRCA1 (pSer988) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 988 of human BRCA1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
double-strand break repair via homologous recombination
transcription coactivator activity
ubiquitin-protein ligase activity
regulation of transcription from RNA polymerase III promoter
fatty acid biosynthetic process
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
zinc ion binding
gamma-tubulin ring complex
DNA damage response, signal transduction resulting in induction of apoptosis
identical protein binding
response to ionizing radiation
positive regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of transcription
transcription activator activity
androgen receptor signaling pathway
positive regulation of protein ubiquitination
G2/M transition DNA damage checkpoint
ubiquitin protein ligase binding
regulation of cell proliferation
response to estrogen stimulus
negative regulation of fatty acid biosynthetic process
positive regulation of DNA repair
negative regulation of centriole replication
androgen receptor binding
cellular response to indole-3-methanol
protein K6-linked ubiquitination
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of BRCA1 (pSer988) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Roy, R. et al. (2011) BRCA1 and BRCA2: different roles in a common pathway of genome protection.
Nat Rev Cancer. 12 (1): 68-78.
Lee, J.S. et al. (2000) hCds1-mediated phosphorylation of BRCA1 regulates the DNA damage response.
Nature. 404 (6774): 201-4.
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