BETA-NGF antibody

100% Secure


Rabbit anti Human BETA-NGF:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2812Z
Datasheet
E WB
SDS
0.1 mg loader

Rabbit anti Human beta-NGF antibody, recognizes beta-Nerve Growth Factor (beta-NGF), a member of the neurotrophin family of proteins. Beta-NGF plays a crucial role in neuronal cell survival and differentiation. It is also critical for the development and maintenance of the nervous system (Yue et al. 2013).

Beta-NGF elicits its biological function through the transmembrane recetprs trkA and p75NTR. Upon activation of these receptors by beta-NGF, a multitude of signaling pathways are stimulated including MAPK, PI3K and ERK, that sensitize neurons and stimulate growth of axons and dendrites (Huang et al. 2018).

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water
Antiserum Preparation
Produced from sera of rabbits immunized with recombinant human beta-NGF and purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
E. coli derived recombinant human beta-NGF
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Endotoxin Level
< 0.1 ng/μg of protein (< 1 EU/μg)

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01138
Entrez Gene
NGF
GO Terms
GO:0000186 activation of MAPKK activity
GO:0006915 apoptosis
GO:0005163 nerve growth factor receptor binding
GO:0005768 endosome
GO:0005796 Golgi lumen
GO:0006916 anti-apoptosis
GO:0007202 activation of phospholipase C activity
GO:0007265 Ras protein signal transduction
GO:0008083 growth factor activity
GO:0008624 induction of apoptosis by extracellular signals
GO:0048015 phosphatidylinositol-mediated signaling
GO:0032455 nerve growth factor processing
GO:0045786 negative regulation of cell cycle
GO:0048011 nerve growth factor receptor signaling pathway
Regulatory
For research purposes only

Applications of BETA-NGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml 2.0 ug/ml
Western Blotting 0.1 ug/ml 0.2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra