BETA-NGF antibody
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WB ResetRabbit anti Human BETA-NGF:Preservative Free
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- BETA-NGF
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Antibody Quality and 
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| Rabbit anti Human beta-NGF antibody, recognizes beta-Nerve Growth Factor (beta-NGF), a member of the neurotrophin family of proteins. Beta-NGF plays a crucial role in neuronal cell survival and differentiation. It is also critical for the development and maintenance of the nervous system (Yue et al. 2013). Beta-NGF elicits its biological function through the transmembrane recetprs trkA and p75NTR. Upon activation of these receptors by beta-NGF, a multitude of signaling pathways are stimulated including MAPK, PI3K and ERK, that sensitize neurons and stimulate growth of axons and dendrites (Huang et al. 2018). |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water
- Antiserum Preparation
- Produced from sera of rabbits immunized with recombinant human beta-NGF and purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human beta-NGF
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Endotoxin Level
- < 0.1 ng/μg of protein (< 1 EU/μg)
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 ug/ml | 2.0 ug/ml |
| Western Blotting | |
0.1 ug/ml | 0.2 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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- UniProt
- P01138
- Entrez Gene
- NGF
- GO Terms
- GO:0000186 activation of MAPKK activity
- GO:0006915 apoptosis
- GO:0005163 nerve growth factor receptor binding
- GO:0005768 endosome
- GO:0005796 Golgi lumen
- GO:0006916 anti-apoptosis
- GO:0007202 activation of phospholipase C activity
- GO:0007265 Ras protein signal transduction
- GO:0008083 growth factor activity
- View More GO Terms
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0032455 nerve growth factor processing
- GO:0045786 negative regulation of cell cycle
- GO:0048011 nerve growth factor receptor signaling pathway
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