Bcl-xL (pSer62) antibody

Rabbit anti Bcl-xL (pSer62)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Bcl-xL (pSer62)

Product Code Applications Pack Size List Price Your Price Qty
AHP2558
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SDS Safety Datasheet SDS
WB 50 µl loader
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Rabbit anti Bcl-xL (pSer62) antibody recognizes Bcl-xL, also known as Bcl-2-like protein 1 (Bcl-2-L-1) and apoptosis regulator Bcl-X, when phosphorylated at serine 62. Bcl-xL is a Bcl-2 family member that has an anti-apoptotic function in the apoptosis signaling cascade.

Bcl-xL inhibits apoptosis by preventing the release of mitochondrial cytochrome c and the subsequent activation of caspases 3 and 9.

Mitotic arrest induces complete phosphorylation of Bcl-xL at serine 62 by cyclin dependent kinase 1 (CDK1), inactivating the anti-apoptotic function of Bcl-xL (Terrano et al. 2010).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Bcl-xL (pSer62) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 62 of human Bcl-xL
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Terrano, D.T. et al. (2010) Cyclin-dependent kinase 1-mediated Bcl-xL/Bcl-2 phosphorylation acts as a functional link coupling mitotic arrest and apoptosis
    Mol Cell Biol. 30 (3): 640-56.

UniProt
Q07817
Entrez Gene
BCL2L1
GO Terms
GO:0001836 release of cytochrome c from mitochondria
GO:0016021 integral to membrane
GO:0005741 mitochondrial outer membrane
GO:0008629 induction of apoptosis by intracellular signals
GO:0008634 negative regulation of survival gene product expression
GO:0042802 identical protein binding
GO:0031965 nuclear membrane
GO:0034097 response to cytokine stimulus
GO:0046902 regulation of mitochondrial membrane permeability
GO:0051881 regulation of mitochondrial membrane potential
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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