Bcl-xL (pSer62) antibody

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Rabbit anti Bcl-xL (pSer62)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2558
Datasheet
WB
SDS
50 µl loader

Rabbit anti Bcl-xL (pSer62) antibody recognizes Bcl-xL, also known as Bcl-2-like protein 1 (Bcl-2-L-1) and apoptosis regulator Bcl-X, when phosphorylated at serine 62. Bcl-xL is a Bcl-2 family member that has an anti-apoptotic function in the apoptosis signaling cascade.

Bcl-xL inhibits apoptosis by preventing the release of mitochondrial cytochrome c and the subsequent activation of caspases 3 and 9.

Mitotic arrest induces complete phosphorylation of Bcl-xL at serine 62 by cyclin dependent kinase 1 (CDK1), inactivating the anti-apoptotic function of Bcl-xL (Terrano et al. 2010).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Bcl-xL (pSer62) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 62 of human Bcl-xL

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q07817
Entrez Gene
BCL2L1
GO Terms
GO:0001836 release of cytochrome c from mitochondria
GO:0016021 integral to membrane
GO:0005741 mitochondrial outer membrane
GO:0008629 induction of apoptosis by intracellular signals
GO:0008634 negative regulation of survival gene product expression
GO:0042802 identical protein binding
GO:0031965 nuclear membrane
GO:0034097 response to cytokine stimulus
GO:0046902 regulation of mitochondrial membrane permeability
GO:0051881 regulation of mitochondrial membrane potential
Regulatory
For research purposes only

Applications of Bcl-xL (pSer62) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Terrano, D.T. et al. (2010) Cyclin-dependent kinase 1-mediated Bcl-xL/Bcl-2 phosphorylation acts as a functional link coupling mitotic arrest and apoptosis
    Mol Cell Biol. 30 (3): 640-56.