Bcl-xL (pSer62) antibody
Rabbit anti Bcl-xL (pSer62)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Bcl-xL (pSer62)
Antibody Quality and Performance Guarantee
| Rabbit anti Bcl-xL (pSer62) antibody recognizes Bcl-xL, also known as Bcl-2-like protein 1 (Bcl-2-L-1) and apoptosis regulator Bcl-X, when phosphorylated at serine 62. Bcl-xL is a Bcl-2 family member that has an anti-apoptotic function in the apoptosis signaling cascade. Bcl-xL inhibits apoptosis by preventing the release of mitochondrial cytochrome c and the subsequent activation of caspases 3 and 9. Mitotic arrest induces complete phosphorylation of Bcl-xL at serine 62 by cyclin dependent kinase 1 (CDK1), inactivating the anti-apoptotic function of Bcl-xL (Terrano et al. 2010). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Bcl-xL (pSer62) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 62 of human Bcl-xL
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Further Reading
-
Terrano, D.T. et al. (2010) Cyclin-dependent kinase 1-mediated Bcl-xL/Bcl-2 phosphorylation acts as a functional link coupling mitotic arrest and apoptosis
Mol Cell Biol. 30 (3): 640-56.
- UniProt
- Q07817
- Entrez Gene
- BCL2L1
- GO Terms
- GO:0001836 release of cytochrome c from mitochondria
- GO:0016021 integral to membrane
- GO:0005741 mitochondrial outer membrane
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008634 negative regulation of survival gene product expression
- GO:0042802 identical protein binding
- GO:0031965 nuclear membrane
- GO:0034097 response to cytokine stimulus
- GO:0046902 regulation of mitochondrial membrane permeability
- View More GO Terms
- GO:0051881 regulation of mitochondrial membrane potential
AHP2558
141520
163802
165056
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