ATM antibody

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Rabbit anti ATM

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2441 WB 50 µl
Rabbit anti ATM antibody recognizes serine-protein kinase ATM, also known as ataxia telangiectasia mutated. ATM is a serine/threonine protein kinase and a member of the PI3K/P14K-kinase family. ATM is a cell cycle checkpoint kinase and is important in the DNA damage response.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ATM was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Synthetic human ATM peptide

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q13315 Related reagents
Entrez Gene
ATM Related reagents
GO Terms
GO:0000724 double-strand break repair via homologous recombination
GO:0002331 pre-B cell allelic exclusion
GO:0003677 DNA binding
GO:0004677 DNA-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006975 DNA damage induced protein phosphorylation
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0007050 cell cycle arrest
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0007131 reciprocal meiotic recombination
GO:0042802 identical protein binding
GO:0016303 1-phosphatidylinositol-3-kinase activity
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0030889 negative regulation of B cell proliferation
GO:0031572 G2/M transition DNA damage checkpoint
GO:0032403 protein complex binding
GO:0043517 positive regulation of DNA damage response, signal transduction by p53 class mediator
GO:0046777 protein autophosphorylation
GO:0046983 protein dimerization activity
GO:0047485 protein N-terminus binding
GO:0071044 histone mRNA catabolic process
GO:0071480 cellular response to gamma radiation
GO:0090399 replicative senescence
Regulatory
For research purposes only

Applications of ATM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti ATM antibody detects a band of approximately 351 kDa in 293T cell lysate under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*