ATG7 antibody
Rabbit anti Human ATG7 antibody (AHP1651) used for the evaluation of ATG7 expression in HeLa cell lysates by western blotting.
Image caption:
Western blot analysis of depletion efficiencies of ATG5 and ATG7 siRNAs. HeLa cells were transfected with siRNAs targeting ATG5, ATG7 or siCtrl for 72 h lysed and probed with antibodies for ATG5 and ATG7 (the asterisk indicates an unspecific band). GAPDH was probed as a loading control.
From: Koerver L, Papadopoulos C, Liu B, et al.
The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage.
EMBO Rep. 2019;20(10):e48014.
doi:10.15252/embr.201948014.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Human ATG7 (C-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- ATG7
- Region
- (C-Terminal)
Antibody Quality and Performance Guarantee
| Rabbit anti Human ATG7 (C-Terminal) antibody recognizes human Autophagy-related protein 7, known also as Ubitiquin Activating Enzyme-E1 like Protein, ATG7, and APG7. ATG7 is a ~78 kDa cytoplasmic protein. In mammalian cells, ATG7 is essential for autophagy conjugation systems, autophagosome formation, and starvation-induced bulk degradation of proteins and organelles. Caspase-8 may alter ATG7 levels and thus the ATG7 program of autophagic cell death (Yu et al. 2004). ATG7 is evolutionarily conserved exhibiting high homology within mammalian species. It expressed in wide range of tissues including kidney, liver, bone marrow and lymph nodes. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human ATG7 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- AHP1651T: <0.1% Sodium Azide (NaN3)
- AHP1651: 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to an 17 amino acid sequence from near the carboxy terminus of human ATG7.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
10ug/ml | |
| Western Blotting | |
1 | 2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1651 detects a band of approximately 78kDa in Caco-2 cell lysate.
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References for ATG7 antibody
-
Pampliega, O. et al. (2013) Functional interaction between autophagy and ciliogenesis.
Nature. 502 (7470): 194-200. -
Koerver, L. et al. (2019) The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage.
EMBO Rep. 20 (10): e48014. -
Pampliega, O. et al. (2021) Amyloid beta oligomers modulate neuronal autophagy through the primary cilium.
BioRχiv 2Jun [Epub ahead of print].
- Synonyms
- UBIQUITIN-ACTIVATING Enzyme E1-LIKE Protein
- RRID
- AB_1898218
- UniProt
- O95352
- Q9D906
- Entrez Gene
- ATG7
- Atg7
- GO Terms
- GO:0004839 ubiquitin activating enzyme activity
- GO:0005737 cytoplasm
- GO:0006497 protein lipidation
- GO:0006914 autophagy
- GO:0006944 cellular membrane fusion
- GO:0015031 protein transport
- GO:0019778 APG12 activating enzyme activity
- GO:0031401 positive regulation of protein modification process
- GO:0042803 protein homodimerization activity
- View More GO Terms
- GO:0005515 protein binding
- GO:0001889 liver development
- GO:0006520 cellular amino acid metabolic process
- GO:0006996 organelle organization
- GO:0050877 neurological system process
- GO:0007628 adult walking behavior
- GO:0009791 post-embryonic development
- GO:0016044 cellular membrane organization
- GO:0021680 cerebellar Purkinje cell layer development
- GO:0021860 pyramidal neuron development
- GO:0021955 central nervous system neuron axonogenesis
- GO:0021987 cerebral cortex development
- GO:0030163 protein catabolic process
- GO:0031396 regulation of protein ubiquitination
- GO:0032446 protein modification by small protein conjugation
- GO:0042594 response to starvation
- GO:0043066 negative regulation of apoptosis
- GO:0055013 cardiac muscle cell development
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