Asymmetric Di-Methyl-Histone H4 (Arg3) antibody
Rabbit anti Asymmetric-Di-Methyl-Histone H4 (Arg3)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Asymmetric Di-Methyl-Histone H4 (Arg3)
Antibody Quality and Performance Guarantee
| Rabbit anti Human asymmetric di-methyl-histone H4 (Arg3) antibody recognizes histone H4 when asymmetrically di-methylated at arginine 3. Histone H4 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones can be mono-, di- or tri-methylated by histone methyltransferases (HMTs). Depending on which amino acid residues are methylated, methylation of histones may increase or decrease the transcription of genes. Methylation events that weaken the binding between histone tails and DNA lead to increased transcription because they make the DNA more accessible to transcription factor proteins and RNA polymerase. Methylation of histones is therefore crucial for the regulation of gene expression (Gupta et al. 2010). Histone H4 may be asymmetrically di-methylated at arginine 3 by the PRMT methyltransferases PRMT1 and PRMT6, an event that normally leads to transcriptional activation by nuclear hormone receptors (Schiza et al. 2013). Wide species cross-reactivity is expected from Rabbit anti Human asymmetric di-methyl-histone H4 (Arg3) antibody based on sequence. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Broad Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to asymmeric-di-methyl-histone H4 (Arg3) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- A synthetic peptide containing asymmetrically di-methylated Arg3 of human histone H4
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoblotting |  |
1/2500 | |
| Western Blotting | |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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- UniProt
- P62805
- Entrez Gene
- HIST2H4B
- GO Terms
- GO:0005515 protein binding
- GO:0000723 telomere maintenance
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0005654 nucleoplasm
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0034080 CenH3-containing nucleosome assembly at centromere
- GO:0045653 negative regulation of megakaryocyte differentiation
AHP3074
160829
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