AKT1 (pSer473) antibody

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WB ResetRabbit anti AKT1 (pSer473)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- AKT1 (pSer473)
Rabbit anti AKT1 (pSer473) antibody recognizes AKT1, also known as RAC-alpha serine/threonine-protein kinase, protein kinase B alpha or Proto-oncogene c-Akt, when phosphorylated at serine 473. AKT1 is a 480 amino acid ~48 kDa protein kinase forming part of the PI3K-Akt signaling pathway, known to regulate cell growth, proliferation and survival. Phosphorylation of threonine 308 and serine 473 are required for full activation of AKT1. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to AKT1 (pSer473) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Proclin™300
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 473 of human AKT1
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- ProClin™ is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | ![]() |
1/500 | 1/2000 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Description | Sheep anti Rabbit IgG:FITC | ||||||
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Description | Sheep anti Rabbit IgG:RPE |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
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Description | TidyBlot Western Blot Detection Reagent:HRP |
- UniProt
- P31749
- Entrez Gene
- AKT1
- GO Terms
- GO:0005886 plasma membrane
- GO:0000060 protein import into nucleus, translocation
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
- GO:0005654 nucleoplasm
- GO:0005978 glycogen biosynthetic process
- GO:0006417 regulation of translation
- View More GO Terms
- GO:0006469 negative regulation of protein kinase activity
- GO:0006809 nitric oxide biosynthetic process
- GO:0006924 activation-induced cell death of T cells
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0008286 insulin receptor signaling pathway
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0042802 identical protein binding
- GO:0009408 response to heat
- GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport
- GO:0010975 regulation of neuron projection development
- GO:0016071 mRNA metabolic process
- GO:0046326 positive regulation of glucose import
- GO:0046889 positive regulation of lipid biosynthetic process
- GO:0019899 enzyme binding
- GO:0018105 peptidyl-serine phosphorylation
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030168 platelet activation
- GO:0030235 nitric-oxide synthase regulator activity
- GO:0030307 positive regulation of cell growth
- GO:0031018 endocrine pancreas development
- GO:0031295 T cell costimulation
- GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S
- GO:0031999 negative regulation of fatty acid beta-oxidation
- GO:0034405 response to fluid shear stress
- GO:0043325 phosphatidylinositol-3,4-bisphosphate binding
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045600 positive regulation of fat cell differentiation
- GO:0045725 positive regulation of glycogen biosynthetic process
- GO:0046777 protein autophosphorylation
- GO:0048009 insulin-like growth factor receptor signaling pathway
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051000 positive regulation of nitric-oxide synthase activity
- GO:0070141 response to UV-A
- GO:0090004 positive regulation of establishment of protein localization in plasma membrane
AHP2544


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