ACTH antibody
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Rabbit anti Human ACTH antibody recognizes human adrenocorticotrophic hormone, also known as corticotrophin. ACTH is a 4.5kDa hormone derived from cleavage of pro-opiomelanocortin (POMC), produced in the pituitary gland in response to corticotrophin releasing hormone (CRH) activity and functions to stimulate release of adrenal cortisol.
Rabbit anti Human ACTH polyclonal antibody is directed against the N-terminal portion of adrenocorticotrophic hormone and also recognizes the ACTH cleavage fragment α-melanocyte stimulating hormone (α-MSH), responsible for melanin pigmentation control in most vertebrate species (Fiordelisio et al. 2006). Rabbit anti Human ACTH does not recognize the C-terminal ACTH cleavage product, corticotropin-like intermediary peptide (CLIP).
Rabbit anti Human ACTH polyclonal antibody is directed against the N-terminal portion of adrenocorticotrophic hormone and also recognizes the ACTH cleavage fragment α-melanocyte stimulating hormone (α-MSH), responsible for melanin pigmentation control in most vertebrate species (Fiordelisio et al. 2006). Rabbit anti Human ACTH does not recognize the C-terminal ACTH cleavage product, corticotropin-like intermediary peptide (CLIP).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Broad Expected from Sequence Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide corresponding to the amino acids 1-24 (SYSMEHFRWGKPVGKKRRPVKVYP) of ACTH conjugated to Keyhole limpet Haemocyanin.
- Approx. Protein Concentrations
- 5.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P01189 Related reagents
- Entrez Gene
- POMC Related reagents
- GO Terms
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0030141 stored secretory granule
- GO:0006091 generation of precursor metabolites and energy
- GO:0007218 neuropeptide signaling pathway
- GO:0007267 cell-cell signaling
- GO:0008217 regulation of blood pressure
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0016486 peptide hormone processing
- GO:0031781 type 3 melanocortin receptor binding
- GO:0031782 type 4 melanocortin receptor binding
- GO:0032098 regulation of appetite
- GO:0032720 negative regulation of tumor necrosis factor production
- GO:0033059 cellular pigmentation
- GO:0034641 cellular nitrogen compound metabolic process
- GO:0042446 hormone biosynthetic process
- GO:0070996 type 1 melanocortin receptor binding
- Regulatory
- For research purposes only
Applications of ACTH antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/1000 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/100 | 1/200 | |
| Immunohistology - Paraffin | 1/100 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- ACTH-containing cells of the anterior pituitary gland.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P |
Product Specific References
References for ACTH antibody
-
Osamura, R.Y. et al. (1980) Adrenocorticotropic hormone cells and immunoreactive beta-endorphin cells in the human pituitary gland: normal and pathologic conditions studied by the peroxidase-labeled antibody method.
Am J Pathol. 99 (1): 105-24. -
Fiordelisio, T. et al. (2006) Cells of proopiomelanocortin lineage from the rodent anterior pituitary lack sexually dimorphic expression of neurofilaments.
Neuroendocrinology. 83:360-70. -
Farris, G.M. et al. (2007) Recombinant rat and mouse growth hormones: risk assessment of carcinogenic potential in 2-year bioassays in rats and mice.
Toxicol Sci. 97: 548-61.
