Rabbit anti Human ACTH antibody
recognizes human adrenocorticotrophic hormone, also known as corticotrophin. ACTH is a 4.5kDa hormone derived from cleavage of pro-opiomelanocortin (POMC), produced in the pituitary gland in response to corticotrophin releasing hormone (CRH) activity and functions to stimulate release of adrenal cortisol.
Rabbit anti Human ACTH polyclonal antibody is directed against the N-terminal portion of adrenocorticotrophic hormone and also recognizes the ACTH cleavage fragment α-melanocyte stimulating hormone (α-MSH), responsible for melanin pigmentation control in most vertebrate species (Fiordelisio et al. 2006
). Rabbit anti Human ACTH does not recognize the C-terminal ACTH cleavage product, corticotropin-like intermediary peptide (CLIP).
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Purified Ig prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Synthetic peptide corresponding to the amino acids 1-24 (SYSMEHFRWGKPVGKKRRPVKVYP) of ACTH conjugated to Keyhole limpet Haemocyanin.
- Approx. Protein Concentrations
- 5.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
stored secretory granule
generation of precursor metabolites and energy
neuropeptide signaling pathway
regulation of blood pressure
positive regulation of gene-specific transcription from RNA polymerase II promoter
peptide hormone processing
type 3 melanocortin receptor binding
type 4 melanocortin receptor binding
regulation of appetite
negative regulation of tumor necrosis factor production
cellular nitrogen compound metabolic process
hormone biosynthetic process
type 1 melanocortin receptor binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ACTH antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- ACTH-containing cells of the anterior pituitary gland.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for ACTH antibody
Osamura, R.Y. et al. (1980) Adrenocorticotropic hormone cells and immunoreactive beta-endorphin cells in the human pituitary gland: normal and pathologic conditions studied by the peroxidase-labeled antibody method.
Am J Pathol. 99 (1): 105-24.
Fiordelisio, T. et al. (2006) Cells of proopiomelanocortin lineage from the rodent anterior pituitary lack sexually dimorphic expression of neurofilaments.
Farris, G.M. et al. (2007) Recombinant rat and mouse growth hormones: risk assessment of carcinogenic potential in 2-year bioassays in rats and mice.
Toxicol Sci. 97: 548-61.