ACTH antibody
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Rabbit anti Human ACTH
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- ACTH
Antibody Quality and Performance Guarantee
| Rabbit anti Human ACTH antibody recognizes human adrenocorticotrophic hormone, also known as corticotrophin. ACTH is a 4.5 kDa hormone derived from cleavage of pro-opiomelanocortin (POMC), produced in the pituitary gland in response to corticotrophin releasing hormone (CRH) activity and functions to stimulate release of adrenal cortisol. Rabbit anti Human ACTH polyclonal antibody is directed against the N-terminal portion of adrenocorticotrophic hormone and also recognizes the ACTH cleavage fragment α-melanocyte stimulating hormone (α-MSH), responsible for melanin pigmentation control in most vertebrate species (Fiordelisio et al. 2006). Rabbit anti Human ACTH does not recognize the C-terminal ACTH cleavage product, corticotropin-like intermediary peptide (CLIP). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Broad Expected from Sequence Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide corresponding to the amino acids 1-24 (SYSMEHFRWGKPVGKKRRPVKVYP) of ACTH conjugated to Keyhole limpet Haemocyanin.
- Approx. Protein Concentrations
- 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/500 | 1/1000 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/100 | 1/200 |
| Immunohistology - Paraffin | |
1/100 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- ACTH-containing cells of the anterior pituitary gland.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
References for ACTH antibody
-
Osamura, R.Y. et al. (1980) Adrenocorticotropic hormone cells and immunoreactive beta-endorphin cells in the human pituitary gland: normal and pathologic conditions studied by the peroxidase-labeled antibody method.
Am J Pathol. 99 (1): 105-24. -
Fiordelisio, T. et al. (2006) Cells of proopiomelanocortin lineage from the rodent anterior pituitary lack sexually dimorphic expression of neurofilaments.
Neuroendocrinology. 83:360-70. -
Farris, G.M. et al. (2007) Recombinant rat and mouse growth hormones: risk assessment of carcinogenic potential in 2-year bioassays in rats and mice.
Toxicol Sci. 97: 548-61.
- RRID
- AB_620352
- UniProt
- P01189
- Entrez Gene
- POMC
- GO Terms
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0030141 stored secretory granule
- GO:0006091 generation of precursor metabolites and energy
- GO:0007218 neuropeptide signaling pathway
- GO:0007267 cell-cell signaling
- GO:0008217 regulation of blood pressure
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0016486 peptide hormone processing
- View More GO Terms
- GO:0031781 type 3 melanocortin receptor binding
- GO:0031782 type 4 melanocortin receptor binding
- GO:0032098 regulation of appetite
- GO:0032720 negative regulation of tumor necrosis factor production
- GO:0033059 cellular pigmentation
- GO:0034641 cellular nitrogen compound metabolic process
- GO:0042446 hormone biosynthetic process
- GO:0070996 type 1 melanocortin receptor binding
0178-0204
160926
166437
1705
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