GFR Alpha 2 antibody
Rabbit anti GFR alpha-2 antibody recognizes the cell surface receptor for neurturin, designated GFR alpha-2. Neurturin is a member of the GDNF family, and has an important role in the control of vertebrate neuron survival and differentiation. GRF alpha-2 is a ~52 kDa glycoprotein, and is a GPI-linked cell surface receptor.
Product Details
- Species Cross-Reactivity
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Target Species Cross Reactivity Rat Human Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human GFR alpha-2 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Synthetic peptide from C-terminus of human GFR alpha-2.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O00451
- Entrez Gene
- GFRA2
- GO Terms
- GO:0005886 plasma membrane
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0031225 anchored to membrane
- GO:0016167 glial cell line-derived neurotrophic factor receptor activity
- GO:0019898 extrinsic to membrane
- Regulatory
- For research purposes only
Applications of GFR Alpha 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Western Blotting | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Kotzbauer, P.T. et al. (1996) Neurturin, a relative of glial-cell-line-derived neurotrophic factor.
Nature. 384 (6608): 467-70. -
Klein, R.D. et al. (1997) A GPI-linked protein that interacts with Ret to form a candidate neurturin receptor.
Nature. 387 (6634): 717-21. -
Buj-bello, A. et al. (1997) Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase.
Nature. 387 (6634): 721-4. -
GFRα Nomenclature Committee (1997) Nomenclature of GPI-linked receptors for the GDNF ligand family.
Neuron 19: 485.
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