IgE antibody

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Goat anti Dog IgE

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IgE

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Secondary Antibodies
References (7)
Applications
Storage Information
Batch/Lot Specific Datasheets

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AHP946 Datasheet Datasheet SDS SDS	E WB	0.5 mg		Log in	Get Quote
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Goat anti Dog IgE antibody recognises canine IgE. The antibody has been shown to react specifically with canine IgE by immunoelectrophoresis and ELISA. Less than 0.01% cross reactivity was detected to canine IgG1, IgG2, IgA and IgM. Goat anti Dog IgE may cross react with IgE from other species.

Goat anti Dog IgE antibody has been reported for use in immunohistology of frozen canine sections.

Target Species: Dog
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to canine IgE were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Immunogen: Native canine IgE.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/100	1/10,000
Western Blotting		1/1000	1/30,000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description	Product Code	Applications	Pack Size	Your Price
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in	Get Quote
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Description	Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in	Get Quote
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Description	Rabbit anti Goat IgG (Fc):HRP

References for IgE antibody

Krachudel, J. et al. (2013) Luteal insufficiency in bitches as a consequence of an autoimmune response against progesterone?
Theriogenology. 79: 1278-83
Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
Vet Immunol Immunopathol. 159: 29-40.
Couto N et al. (2016) Identification of vaccine candidate antigens of Staphylococcus pseudintermedius by whole proteome characterization and serological proteomic analyses.
J Proteomics. 133: 113-24.
Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
Vet Immunol Immunopathol. 159 (1-2): 29-40.
Moya, R.et al. (2016) Immunoproteomic characterization of a Dermatophagoides farinae extract used in the treatment of canine atopic dermatitis
Vet Immunol and Immunopathol. Aug 4 [Epub ahead of print]
Wysmołek, M.E. et al. (2022) Canine antibody response against Dirofilaria repens. in natural occult and microfilaremic infections
Comp Immunol Microbiol Infect Dis. 2 May: 101818 [Epub ahead of print].
Bizikova, P. et al. (2014) Serum autoantibody profiles of IgA, IgE and IgM in canine pemphigus foliaceus.
Vet Dermatol. 25 (5): 471-e75.