IgE antibody

Goat anti Dog IgE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgE

Product Code Applications Pack Size List Price Your Price Qty
AHP946
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.5 mg loader
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loader
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Goat anti Dog IgE antibody recognises canine IgE. The antibody has been shown to react specifically with canine IgE by immunoelectrophoresis and ELISA. Less than 0.01% cross reactivity was detected to canine IgG1, IgG2, IgA and IgM. Goat anti Dog IgE may cross react with IgE from other species.

Goat anti Dog IgE antibody has been reported for use in immunohistology of frozen canine sections.

Target Species
Dog
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to canine IgE were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Native canine IgE.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/10,000
Western Blotting 1/1000 1/30,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IgE antibody

  1. Krachudel, J. et al. (2013) Luteal insufficiency in bitches as a consequence of an autoimmune response against progesterone?
    Theriogenology. 79: 1278-83
  2. Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
    Vet Immunol Immunopathol. 159: 29-40.
  3. Couto N et al. (2016) Identification of vaccine candidate antigens of Staphylococcus pseudintermedius by whole proteome characterization and serological proteomic analyses.
    J Proteomics. 133: 113-24.
  4. Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
    Vet Immunol Immunopathol. 159 (1-2): 29-40.
  5. Moya, R.et al. (2016) Immunoproteomic characterization of a Dermatophagoides farinae extract used in the treatment of canine atopic dermatitis
    Vet Immunol and Immunopathol. Aug 4 [Epub ahead of print]
  6. Wysmołek, M.E. et al. (2022) Canine antibody response against Dirofilaria repens. in natural occult and microfilaremic infections
    Comp Immunol Microbiol Infect Dis. 2 May: 101818 [Epub ahead of print].
  7. Bizikova, P. et al. (2014) Serum autoantibody profiles of IgA, IgE and IgM in canine pemphigus foliaceus.
    Vet Dermatol. 25 (5): 471-e75.

RRID
AB_567015
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Dog Products