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IgA antibody

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Anti Dog IgA Antibody gallery image 1
Published customer image:
Goat anti Dog IgA antibody used for the measurement of serum IgA levels in dogs with atopic dermatitis by ELISA.
Image caption:
Correlation between the phenotypes and obvious population structure was detected in the GSD population. The difference in IgA levels (pdiff = 1.1×10−5, based on Welsh two sample t-test) in CAD cases and CAD controls (Ntotal = 184, before removing CAD controls with low IgA levels) is presented with boxplots (A). The GSD population is visualized with an MDS-plot displaying the formation of two subpopulations (B) with the uneven distribution of CAD cases and CAD controls (C). The distribution of IgA levels across the subpopulations is visualized with violin plots (D). Panels B-D include all dogs after QC (n = 203) and the dotted lines on the violin plots (A and D) correspond to the respective median values.

From: Tengvall K, Kierczak M, Bergvall K, Olsson M, Frankowiack M, et al. (2013)
Genome-Wide Analysis in German Shepherd Dogs Reveals Association of a Locus on CFA 27 with Atopic Dermatitis.
PLoS Genet 9(5): e1003475.

Goat anti Dog IgA:FITC

Goat anti Dog IgA:HRP

Goat anti Dog IgA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAI31F C F 1 mg
AAI31P C E 1 mg
AAI31 C E ID WB 1 mg
Goat anti Dog IgA polyclonal antibody recognizes canine IgA and shows no cross reactivity with other canine immunoglobulin classes in immunoelectrophoresis or ELISA.

Goat anti Dog IgA may cross react with IgA from other species. Goat anti Dog IgA polyclonal antibody has been shown to cross react with IgA from other canids (Frankowiack et al. 2013) and with mustelids (Martel et al. 2009).

Product Details

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mustelid Expected from Sequence
Ferret
Wolf
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - Liquid
Antiserum Preparation
Antisera to canine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.05% Proclin™ 300
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified canine IgA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.

More Information

Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1/50 1/500
ELISA 1/10,000 1/80,000
Immunohistology - Frozen 1/200 1/500
ELISA
Immunodiffusion
Immunohistology - Frozen 1/200 1/500
Western Blotting Reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

ELISA

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for IgA antibody

  1. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.
    J Nutr. 133 (4): 1158-62.
  2. Peters, I.R. et al. (2004) Measurement of immunoglobulin concentrations in the feces of healthy dogs.
    Clin Diagn Lab Immunol. 11 (5): 841-8.
  3. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  4. Simpson, K.W. et al. (2009) Influence of Enterococcus faecium SF68 probiotic on giardiasis in dogs.
    J Vet Intern Med. 23: 476-81.
  5. Huang, S.S. et al. (2012) Differential Pathological and Immune Responses in Newly Weaned Ferrets Are Associated with a Mild Clinical Outcome of Pandemic 2009 H1N1 Infection.
    J Virol. 86: 13187-201.
  6. Frankowiack, M. et al. (2013) IgA deficiency in wolves.
    Dev Comp Immunol. 40 (2): 180-4.
  7. Olsson M et al. (2014) The dog as a genetic model for immunoglobulin A (IgA) deficiency: identification of several breeds with low serum IgA concentrations.
    Vet Immunol Immunopathol. 160 (3-4): 255-9.
  8. Frankowiack, M. et al. (2015) IgA deficiency in wolves from Canada and Scandinavia.
    Dev Comp Immunol. 50 (1): 26-8.
  9. Lee, A. et al. (2015) Lack of correlation between mucosal immunoglobulin A-positive plasma cell numbers and TLR5 genotypes in German shepherd dogs with idiopathic chronic enteropathy.
    J Comp Pathol. 152 (2-3): 201-5.
  10. Tengvall, K. et al. (2013) Genome-wide analysis in German shepherd dogs reveals association of a locus on CFA 27 with atopic dermatitis.
    PLoS Genet. 9 (5): e1003475.
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