IgA antibody
Goat anti Dog IgA antibody used for the measurement of serum IgA levels in dogs with atopic dermatitis by ELISA.
Image caption:
Correlation between the phenotypes and obvious population structure was detected in the GSD population. The difference in IgA levels (pdiff = 1.1×10−5, based on Welsh two sample t-test) in CAD cases and CAD controls (Ntotal = 184, before removing CAD controls with low IgA levels) is presented with boxplots (A). The GSD population is visualized with an MDS-plot displaying the formation of two subpopulations (B) with the uneven distribution of CAD cases and CAD controls (C). The distribution of IgA levels across the subpopulations is visualized with violin plots (D). Panels B-D include all dogs after QC (n = 203) and the dotted lines on the violin plots (A and D) correspond to the respective median values.
From: Tengvall K, Kierczak M, Bergvall K, Olsson M, Frankowiack M, et al. (2013)
Genome-Wide Analysis in German Shepherd Dogs Reveals Association of a Locus on CFA 27 with Atopic Dermatitis.
PLoS Genet 9(5): e1003475.
Sheep anti Dog IgA polyclonal antibody (AAI31F) used to stain bacteria carryiing canine IgA by flow cytometry.
Image caption:
Representative staining of CE during active disease and remission.
For microbial fraction identification the trigger was set up based on side scatter properties. Then, singlets were selected based on SSC-width vs SCC-height. FITC-negative window population was set up in samples containing only SYTO 17 (A). These windows were applied to samples stained with FITC-IgA (B) or FITC-IgG (C) to distinguish the negative population from the positive one.
From: Martínez-López LM, Perez-Gonzalez A, Washington EA, Woodward AP, Roth-Schulze AJ, Dandrieux JRS, et al. (2021)
Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
PLoS ONE 16(8): e0255012.
doi:10.1371/journal.pone.0255012
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Dog IgA antibody, clone K117 1A8 (MCA631GA) used as a capture reagent in a sandwich ELISA with HRP conjugated anti canine IgA (AAI31P) as a detection reagent.
Image caption:
Immunoglobulin IgA titers in fecal contents collected at the beginning of the study (T0), after 2 weeks (T2), 4 weeks (T4), 6 weeks (T6), and 8 weeks (T8) in dogs fed the same diet with (Group A) or without Slab51® probiotic supplement (Group B). Black hashtags indicate statistical significance between groups. Red asterisks indicate statistical significance by study times within group A.
From: Rossi G, Pengo G, Galosi L, Berardi S, Tambella AM, Attili AR, Gavazza A, Cerquetella M, Jergens AE, Guard BC, Lidbury JA, Stainer JM, Crovace AM and Suchodolski JS (2020)
Effects of the Probiotic Mixture Slab51® (SivoMixx®) as Food Supplement in Healthy Dogs: Evaluation of Fecal Microbiota, Clinical Parameters and Immune Function.
Front. Vet. Sci. 7:613.
doi: 10.3389/fvets.2020.00613
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
HRP conjugated Goat anti canine IgA antibody (AAI31P) used as a detection reagent in a sandwich ELISA with Mouse anti Canine IgA antibody, clone K117 1A8 ( MCA631GA) as a capture reagent.
Image caption:
GALT response: Changes in fecal IgA in each group during the trial are depicted as mean fecal IgA values ± SEM. X-axis depicts time in weeks and Y-axis depicts mean fecal IgA values in μg/ml. #p <0.05.
From: Satyaraj E, Reynolds A, Engler R, Labuda J, Sun P.
Supplementation of Diets With Spirulina Influences Immune and Gut Function in Dogs.
Front Nutr. 2021 May 28;8:667072.
doi: 10.3389/fnut.2021.667072.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Dog IgA polyclonal antibody recognizes canine IgA and shows no cross reactivity with other canine immunoglobulin classes in immunoelectrophoresis or ELISA.
Goat anti Dog IgA may cross react with IgA from other species. Goat anti Dog IgA polyclonal antibody has been shown to cross react with IgA from other canids (Frankowiack et al. 2013) and with mustelids (Martel et al. 2009).
Goat anti Dog IgA may cross react with IgA from other species. Goat anti Dog IgA polyclonal antibody has been shown to cross react with IgA from other canids (Frankowiack et al. 2013) and with mustelids (Martel et al. 2009).
Product Details
- Target Species
- Dog
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mustelid Expected from Sequence Ferret Wolf - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - Liquid
- Antiserum Preparation
- Antisera to canine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Preservative Stabilisers
- 0.05% Proclin™ 300
0.2% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Purified canine IgA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen | 1/50 | 1/500 | |
| ELISA | 1/10,000 | 1/80,000 | |
| Immunohistology - Frozen | 1/200 | 1/500 | |
| ELISA | 1/100 | 1/30000 | |
| Immunodiffusion | |||
| Immunohistology - Frozen | 1/200 | 1/2000 | |
| Western Blotting | Reducing conditions |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgA antibody
-
Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.
J Nutr. 133 (4): 1158-62. -
Peters, I.R. et al. (2004) Measurement of immunoglobulin concentrations in the feces of healthy dogs.
Clin Diagn Lab Immunol. 11 (5): 841-8. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Simpson, K.W. et al. (2009) Influence of Enterococcus faecium SF68 probiotic on giardiasis in dogs.
J Vet Intern Med. 23: 476-81. -
Huang, S.S. et al. (2012) Differential Pathological and Immune Responses in Newly Weaned Ferrets Are Associated with a Mild Clinical Outcome of Pandemic 2009 H1N1 Infection.
J Virol. 86: 13187-201. -
Frankowiack, M. et al. (2013) IgA deficiency in wolves.
Dev Comp Immunol. 40 (2): 180-4. -
Olsson M et al. (2014) The dog as a genetic model for immunoglobulin A (IgA) deficiency: identification of several breeds with low serum IgA concentrations.
Vet Immunol Immunopathol. 160 (3-4): 255-9. -
Frankowiack, M. et al. (2015) IgA deficiency in wolves from Canada and Scandinavia.
Dev Comp Immunol. 50 (1): 26-8. -
Lee, A. et al. (2015) Lack of correlation between mucosal immunoglobulin A-positive plasma cell numbers and TLR5 genotypes in German shepherd dogs with idiopathic chronic enteropathy.
J Comp Pathol. 152 (2-3): 201-5. -
Tengvall, K. et al. (2013) Genome-wide analysis in German shepherd dogs reveals association of a locus on CFA 27 with atopic dermatitis.
PLoS Genet. 9 (5): e1003475. -
Martínez-López, L.M. et al. (2021) Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
PLoS One. 16 (8): e0255012. -
Sfacteria, A. et al. (2021) Immune Cells and Immunoglobulin Expression in the Mammary Gland Tumors of Dog.
Animals (Basel). 11(5):1189. -
Rossi, G. et al. (2020) Effects of the Probiotic Mixture Slab51® (SivoMixx®) as Food Supplement in Healthy Dogs: Evaluation of Fecal Microbiota, Clinical Parameters and Immune Function.
Front Vet Sci. 7: 613. -
Satyaraj, E. et al. (2021) Supplementation of Diets With Spirulina Influences Immune and Gut Function in Dogs.
Front Nutr. 8: 667072.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
