IgA antibody
Goat anti Dog IgA may cross react with IgA from other species. Goat anti Dog IgA polyclonal antibody has been shown to cross react with IgA from other canids (Frankowiack et al. 2013) and with mustelids (Martel et al. 2009).
Product Details
- Target Species
- Dog
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mustelid Expected from Sequence Ferret Wolf - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - Liquid
- Antiserum Preparation
- Antisera to canine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Preservative Stabilisers
- 0.05% Proclin™ 300
0.2% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Purified canine IgA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgA antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | |||
Immunohistology - Frozen | 1/50 | 1/500 | |
ELISA | 1/10,000 | 1/80,000 | |
Immunohistology - Frozen | 1/200 | 1/500 | |
ELISA | 1/100 | 1/30000 | |
Immunodiffusion | |||
Immunohistology - Frozen | 1/200 | 1/2000 | |
Western Blotting | Reducing conditions |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgA antibody
-
Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.
J Nutr. 133 (4): 1158-62. -
Peters, I.R. et al. (2004) Measurement of immunoglobulin concentrations in the feces of healthy dogs.
Clin Diagn Lab Immunol. 11 (5): 841-8. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Simpson, K.W. et al. (2009) Influence of Enterococcus faecium SF68 probiotic on giardiasis in dogs.
J Vet Intern Med. 23: 476-81. -
Huang, S.S. et al. (2012) Differential Pathological and Immune Responses in Newly Weaned Ferrets Are Associated with a Mild Clinical Outcome of Pandemic 2009 H1N1 Infection.
J Virol. 86: 13187-201. -
Frankowiack, M. et al. (2013) IgA deficiency in wolves.
Dev Comp Immunol. 40 (2): 180-4. -
Olsson M et al. (2014) The dog as a genetic model for immunoglobulin A (IgA) deficiency: identification of several breeds with low serum IgA concentrations.
Vet Immunol Immunopathol. 160 (3-4): 255-9. -
Frankowiack, M. et al. (2015) IgA deficiency in wolves from Canada and Scandinavia.
Dev Comp Immunol. 50 (1): 26-8. -
Lee, A. et al. (2015) Lack of correlation between mucosal immunoglobulin A-positive plasma cell numbers and TLR5 genotypes in German shepherd dogs with idiopathic chronic enteropathy.
J Comp Pathol. 152 (2-3): 201-5. -
Tengvall, K. et al. (2013) Genome-wide analysis in German shepherd dogs reveals association of a locus on CFA 27 with atopic dermatitis.
PLoS Genet. 9 (5): e1003475.
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