IgA antibody

Goat anti Dog IgA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgA

Product Code Applications Pack Size List Price Your Price Qty
AAI31
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SDS Safety Datasheet SDS
C E ID WB 1 mg loader
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Goat anti Dog IgA polyclonal antibody recognizes canine IgA and shows no cross reactivity with other canine immunoglobulin classes in immunoelectrophoresis or ELISA.

Goat anti Dog IgA may cross react with IgA from other species. Goat anti Dog IgA polyclonal antibody has been shown to cross react with IgA from other canids (Frankowiack et al. 2013) and with mustelids (Martel et al. 2009).

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mustelid Expected from Sequence
Ferret
Wolf
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Antiserum Preparation
Antisera to canine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified canine IgA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/30000
Immunodiffusion
Immunohistology - Frozen 1/200 1/2000
Western Blotting Reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

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References for IgA antibody

  1. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.
    J Nutr. 133 (4): 1158-62.
  2. Peters, I.R. et al. (2004) Measurement of immunoglobulin concentrations in the feces of healthy dogs.
    Clin Diagn Lab Immunol. 11 (5): 841-8.
  3. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  4. Simpson, K.W. et al. (2009) Influence of Enterococcus faecium SF68 probiotic on giardiasis in dogs.
    J Vet Intern Med. 23: 476-81.
  5. Huang, S.S. et al. (2012) Differential Pathological and Immune Responses in Newly Weaned Ferrets Are Associated with a Mild Clinical Outcome of Pandemic 2009 H1N1 Infection.
    J Virol. 86: 13187-201.
  6. Frankowiack, M. et al. (2013) IgA deficiency in wolves.
    Dev Comp Immunol. 40 (2): 180-4.
  7. Olsson M et al. (2014) The dog as a genetic model for immunoglobulin A (IgA) deficiency: identification of several breeds with low serum IgA concentrations.
    Vet Immunol Immunopathol. 160 (3-4): 255-9.
  8. Frankowiack, M. et al. (2015) IgA deficiency in wolves from Canada and Scandinavia.
    Dev Comp Immunol. 50 (1): 26-8.
  9. View The Latest Product References
  10. Lee, A. et al. (2015) Lack of correlation between mucosal immunoglobulin A-positive plasma cell numbers and TLR5 genotypes in German shepherd dogs with idiopathic chronic enteropathy.
    J Comp Pathol. 152 (2-3): 201-5.
  11. Tengvall, K. et al. (2013) Genome-wide analysis in German shepherd dogs reveals association of a locus on CFA 27 with atopic dermatitis.
    PLoS Genet. 9 (5): e1003475.
  12. Martínez-López, L.M. et al. (2021) Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
    PLoS One. 16 (8): e0255012.
  13. Sfacteria, A. et al. (2021) Immune Cells and Immunoglobulin Expression in the Mammary Gland Tumors of Dog.
    Animals (Basel). 11(5):1189.
  14. Rossi, G. et al. (2020) Effects of the Probiotic Mixture Slab51® (SivoMixx®) as Food Supplement in Healthy Dogs: Evaluation of Fecal Microbiota, Clinical Parameters and Immune Function.
    Front Vet Sci. 7: 613.
  15. Satyaraj, E. et al. (2021) Supplementation of Diets With Spirulina Influences Immune and Gut Function in Dogs.
    Front Nutr. 8: 667072.

ELISA

Immunohistology - Paraffin

Western Blotting

RRID
AB_323059
View more products with IGA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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